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GATC Polyclonal antibody

GATC Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23183-1-AP

Print datasheet

Synonyms

15E1.2, GATC, GatC like protein, Protein 15E1.2

验证数据展示

  • IHC staining of human liver using 23183-1-AP

    IHC staining of human liver using 23183-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23183-1-AP (GATC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 23183-1-AP

    IHC staining of human liver using 23183-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23183-1-AP (GATC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human appendicitis using 23183-1-AP

    IHC staining of human appendicitis using 23183-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 23183-1-AP (GATC antibody) at dilution of 1:400 (under 10x lens).

  • IHC staining of human appendicitis using 23183-1-AP

    IHC staining of human appendicitis using 23183-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 23183-1-AP (GATC antibody) at dilution of 1:400 (under 40x lens).

  • IP experiment of mouse liver using 23183-1-AP

    IP experiment of mouse liver using 23183-1-AP

    IP result of anti-GATC (IP:23183-1-AP, 3ug; Detection:23183-1-AP 1:500) with mouse liver tissue lysate 12500ug.

经过测试的应用

Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue, human appendicitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23183-1-AP targets GATC in IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IP, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen GATC fusion protein Ag19713 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glutamyl-tRNA(Gln) amidotransferase, subunit C homolog (bacterial)
Synonyms 15E1.2, GATC, GatC like protein, Protein 15E1.2
Calculated Molecular Weight 136 aa, 15 kDa
Observed Molecular Weight 15 kDa
GenBank Accession NumberBC107145
Gene Symbol GATC
Gene ID (NCBI) 283459
RRIDAB_2879226
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDO43716
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GATC encodes a subunit of the glutamyl-tRNA(Gln) amidotransferase protein complex (GatCAB), which plays a role in aminoacylation of mitochondrial glutaminyl mt-tRNA. The complex plays an role in mitochondrial translation and protein synthesis (PubMed: 30283131).

实验方案

Product Specific Protocols
IHC protocol for GATC antibody 23183-1-APDownload protocol
IP protocol for GATC antibody 23183-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols