验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, human kidney tissue, rat kidney tissue, rat pancreas tissue |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12801-1-AP targets GATM in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | GATM fusion protein Ag3754 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | glycine amidinotransferase (L-arginine:glycine amidinotransferase) |
| Synonyms | AGAT, AT, GATM, Transamidinase |
| Calculated Molecular Weight | 423 aa, 48 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC004141 |
| Gene Symbol | GATM |
| Gene ID (NCBI) | 2628 |
| RRID | AB_2109060 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P50440 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GATM, also known as AGAT, produces ornithine and guanidinoacetate (GAA) from arginine and glycine, and is a key enzyme for creatine synthesis. GATM deficiency in early infancy causes neurodevelopmental delay. GATM is predominantly expressed in kidney and pancreas in adults. Decrease of GAMT proteins was observed in proximal tubule damage cases, which links GATM as a general marker for proximal tubule damage. Alternative splicing of GATM generates three isoforms with molecular weights between 44 kDa to 48 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GATM antibody 12801-1-AP | Download protocol |
| IHC protocol for GATM antibody 12801-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab Lighting up arginine metabolism reveals its functional diversity in physiology and pathology | ||
Biomed Pharmacother Metabolomic profiling of metoprolol-induced cardioprotection in a murine model of acute myocardial ischemia. | ||
J Agric Food Chem Artepillin C, a key component of Brazilian propolis, induces thermogenesis in inguinal white adipose tissue of mice through a creatine metabolism-related thermogenic pathway. | ||
Gene Clinical and functional characterization of rare compound heterozygous mutations in the SERPINC1 gene causing severe thrombophilia | ||
Front Physiol Arginine:Glycine Amidinotransferase Is Essential for Creatine Supply in Mice During Chronic Hypoxia. | ||
Front Nutr Influence of homoarginine on creatine accumulation and biosynthesis in the mouse |
