验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, human kidney tissue, rat kidney tissue, rat pancreas tissue |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12801-1-AP targets GATM in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | GATM fusion protein Ag3754 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glycine amidinotransferase (L-arginine:glycine amidinotransferase) |
| 别名 | AGAT, AT, GATM, Transamidinase |
| 计算分子量 | 423 aa, 48 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | BC004141 |
| 基因名称 | GATM |
| Gene ID (NCBI) | 2628 |
| RRID | AB_2109060 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P50440 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GATM, also known as AGAT, produces ornithine and guanidinoacetate (GAA) from arginine and glycine, and is a key enzyme for creatine synthesis. GATM deficiency in early infancy causes neurodevelopmental delay. GATM is predominantly expressed in kidney and pancreas in adults. Decrease of GAMT proteins was observed in proximal tubule damage cases, which links GATM as a general marker for proximal tubule damage. Alternative splicing of GATM generates three isoforms with molecular weights between 44 kDa to 48 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GATM antibody 12801-1-AP | Download protocol |
| IHC protocol for GATM antibody 12801-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab Lighting up arginine metabolism reveals its functional diversity in physiology and pathology | ||
Biomed Pharmacother Metabolomic profiling of metoprolol-induced cardioprotection in a murine model of acute myocardial ischemia. | ||
J Agric Food Chem Artepillin C, a key component of Brazilian propolis, induces thermogenesis in inguinal white adipose tissue of mice through a creatine metabolism-related thermogenic pathway. | ||
Gene Clinical and functional characterization of rare compound heterozygous mutations in the SERPINC1 gene causing severe thrombophilia | ||
Front Physiol Arginine:Glycine Amidinotransferase Is Essential for Creatine Supply in Mice During Chronic Hypoxia. | ||
Front Nutr Influence of homoarginine on creatine accumulation and biosynthesis in the mouse |
