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GBA Polyclonal antibody

GBA Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27972-1-AP

Print datasheet

Synonyms

Acid beta glucosidase, Alglucerase, Beta glucocerebrosidase, GBA, GBA1, GC, GCB, GLUC, Glucosylceramidase, Imiglucerase

验证数据展示

  • WB analysis using 27972-1-AP

    WB analysis using 27972-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27972-1-AP (GBA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 27972-1-AP

    IHC staining of mouse brain using 27972-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27972-1-AP (GBA antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 27972-1-AP

    IHC staining of mouse brain using 27972-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27972-1-AP (GBA antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inSH-SY5Y cells, HeLa cells
Positive IHC detected inmouse brain tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

27972-1-AP targets GBA in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, Mouse
Cited Reactivityhuman, mouse
Immunogen GBA fusion protein Ag27388 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glucosidase, beta; acid (includes glucosylceramidase)
Synonyms Acid beta glucosidase, Alglucerase, Beta glucocerebrosidase, GBA, GBA1, GC, GCB, GLUC, Glucosylceramidase, Imiglucerase
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60-65 kDa
GenBank Accession NumberBC003356
Gene Symbol GBA
Gene ID (NCBI) 2629
RRIDAB_2881024
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04062
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GBA antibody 27972-1-APDownload protocol
IHC protocol for GBA antibody 27972-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell Proteomics

Multi Cell Line Analysis of Lysosomal Proteomes Reveals Unique Features and Novel Lysosomal Proteins

Authors - Fatema Akter
View Article
mouseWB

Sci Total Environ

Manganese disrupts the maturation and degradation of axonal autophagosome leading to hippocampal synaptic toxicity in mice via the activation of LRRK2 on phosphorylation of Rab10

Authors - Zhuo Ma
View Article