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验证数据展示

  • IHC staining of human breast cancer using 20622-1-AP

    IHC staining of human breast cancer using 20622-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 20622-1-AP (GBA antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human breast cancer using 20622-1-AP

    IHC staining of human breast cancer using 20622-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 20622-1-AP (GBA antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of SH-SY5Y using 20622-1-AP

    IF Staining of SH-SY5Y using 20622-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 20622-1-AP (GBA antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

GBA Polyclonal antibody

GBA Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 20622-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

GBA1LP, glucosylceramidase beta 1 like, pseudogene, GBAP1, GBAP, Beta-GC

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman breast cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20622-1-AP targets GBA in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 GBA fusion protein Ag13981 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glucosidase, beta; acid, pseudogene
别名 GBA1LP, glucosylceramidase beta 1 like, pseudogene, GBAP1, GBAP, Beta-GC
计算分子量 321 aa, 36 kDa
GenBank蛋白编号BC000349
基因名称 GBA
Gene ID (NCBI) 2630
RRIDAB_10696317
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for GBA antibody 20622-1-APDownload protocol
IF protocol for GBA antibody 20622-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols