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- KD/KO Validated
GBF1 Polyclonal antibody
GBF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of 25183-1-AP is GBF1 Fusion Protein expressed in E. coli.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | GBF1 fusion protein Ag16579 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | golgi-specific brefeldin A resistant guanine nucleotide exchange factor 1 |
| Synonyms | ARF1GEF, BFA resistant GEF 1, GBF1, KIAA0248 |
| Calculated Molecular Weight | 1859 aa, 206 kDa |
| Observed Molecular Weight | 206 kDa |
| GenBank Accession Number | BC117681 |
| Gene Symbol | GBF1 |
| Gene ID (NCBI) | 8729 |
| RRID | AB_2879948 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92538 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GBF1 antibody 25183-1-AP | Download protocol |
| IHC protocol for GBF1 antibody 25183-1-AP | Download protocol |
| IF protocol for GBF1 antibody 25183-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
PLoS Pathog Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication. | ||
Sci Rep The Golgi protein ACBD3 facilitates Enterovirus 71 replication by interacting with 3A.
| ||
J Virol Enterovirus 3A protein disrupts endoplasmic reticulum homeostasis through interaction with GBF1 |