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GBP2 Monoclonal antibody

GBP2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2D7G4

Cat no : 68559-1-Ig

Print datasheet

Synonyms

GBP 2, GBP2, GTP binding protein 2, HuGBP 2

验证数据展示

  • WB analysis using 68559-1-Ig

    WB analysis using 68559-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68559-1-Ig (GBP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHT-1376 cells, Saos-2 cells, HepG2 cells, PC-3 cells, A375 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68559-1-Ig targets GBP2 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen GBP2 fusion protein Ag24108 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name guanylate binding protein 2, interferon-inducible
Synonyms GBP 2, GBP2, GTP binding protein 2, HuGBP 2
Calculated Molecular Weight 591 aa, 67 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC022272
Gene Symbol GBP2
Gene ID (NCBI) 2634
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP32456
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GBP2 is a member of the GTPase family and is crucial to host immunity against pathogens. GBP2 is also used as a marker of interferon (IFN) responsiveness, because it is one of the most highly expressed genes after IFN-γ stimulation. Increased GBP2 expression is also associated with a better prognosis in breast cancer and may have a role in T-cell defense against breast cancer. Interestingly, GBP2 expression in NIH/3T3 fibroblasts inhibited Rac activation and matrix metalloproteinase-9 expression, suggesting a possible role for GBP2 in regulating cancer metastasis. The molecular weight of GBP2 is 67 kDa. (PMID: 29072687, 35383115)

实验方案

Product Specific Protocols
WB protocol for GBP2 antibody 68559-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols