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GBP3 Polyclonal antibody

GBP3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30719-1-AP

Print datasheet

Synonyms

Guanine nucleotide-binding protein 3, GTP-binding protein 3, GTP binding protein 3, GBP-3, GBP 3

验证数据展示

  • WB analysis using 30719-1-AP

    WB analysis using 30719-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30719-1-AP (GBP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 30719-1-AP

    IHC staining of mouse cerebellum using 30719-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 30719-1-AP (GBP3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GBP3 antibody included
  • IHC staining of mouse lung using 30719-1-AP

    IHC staining of mouse lung using 30719-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30719-1-AP (GBP3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GBP3 antibody included

经过测试的应用

Positive WB detected inHUVEC cells
Positive IHC detected inmouse cerebellum tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy GBP3 Ready-To-Use IHC Kit. GBP3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30719-1-AP targets GBP3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen GBP3 fusion protein Ag33889 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name guanylate binding protein 3
Synonyms Guanine nucleotide-binding protein 3, GTP-binding protein 3, GTP binding protein 3, GBP-3, GBP 3
Observed Molecular Weight68 kDa
GenBank Accession NumberBC140837
Gene Symbol GBP3
Gene ID (NCBI) 2635
RRIDAB_3086400
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H0R5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Guanylate-binding protein 3 (GBP3) is involved in the proliferation of glioma cells through regulating SQSTM1-ERK1/2 pathway. GBP3 has 2 isoforms, one is 595 amino acids long at 68 kDa and the other is at nearly 62 kDa.

实验方案

Product Specific Protocols
WB protocol for GBP3 antibody 30719-1-APDownload protocol
IHC protocol for GBP3 antibody 30719-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols