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GBP3 Recombinant antibody

GBP3 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230178D8

Cat no : 83534-1-RR

Print datasheet

Synonyms

GTP-binding protein 3, GTP binding protein 3, GBP-3, GBP 3, EC:3.6.5.-

验证数据展示

  • WB analysis using 83534-1-RR

    WB analysis using 83534-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83534-1-RR (GBP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of HT-29 using 83534-1-RR

    FC experiment of HT-29 using 83534-1-RR

    1x10^6 HT-29 cells were intracellularly stained with 0.25 ug Gbp3 Recombinant Antibody (83534-1-RR, Clone:230178D8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83534-1-RR

    Affinity and Kinetic Characterization of 83534-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83534-1-RR against Human GBP3 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inK-562 cells, HeLa cells, Jurkat cells, HUVEC cells, U-251 cells
Positive FC (Intra) detected inHT-29 cells
Planning an IHC experiment? We recommend our IHCeasy GBP3 Ready-To-Use IHC Kit. GBP3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83534-1-RR targets GBP3 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen GBP3 fusion protein Ag33889 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name guanylate binding protein 3
Synonyms GTP-binding protein 3, GTP binding protein 3, GBP-3, GBP 3, EC:3.6.5.-
Observed Molecular Weight62 kDa, 68 kDa
GenBank Accession NumberBC140837
Gene Symbol GBP3
Gene ID (NCBI) 2635
RRIDAB_3671159
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9H0R5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Guanylate-binding protein 3 (GBP3) is involved in the proliferation of glioma cells through regulating SQSTM1-ERK1/2 pathway. GBP3 has 2 isoforms, one is 595 amino acids long at 68 kDa and the other is at nearly 62 kDa.

实验方案

Product Specific Protocols
WB protocol for GBP3 antibody 83534-1-RRDownload protocol
FC protocol for GBP3 antibody 83534-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols