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验证数据展示

  • WB analysis of HeLa using 23001-1-AP

    WB analysis of HeLa using 23001-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23001-1-AP (GBP6 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 23001-1-AP

    IHC staining of human testis using 23001-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23001-1-AP (GBP6 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 23001-1-AP

    IHC staining of human testis using 23001-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23001-1-AP (GBP6 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human lung cancer using 23001-1-AP

    IHC staining of human lung cancer using 23001-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 23001-1-AP (GBP6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GBP6 Polyclonal antibody

GBP6 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 23001-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Guanylate-binding protein 6, Guanine nucleotide-binding protein 6, GTP-binding protein 6, GBP-6, EC:3.6.5.-

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells
Positive IHC detected inhuman testis tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

23001-1-AP targets GBP6 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 GBP6 fusion protein Ag19196 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 guanylate binding protein family, member 6
别名 Guanylate-binding protein 6, Guanine nucleotide-binding protein 6, GTP-binding protein 6, GBP-6, EC:3.6.5.-
计算分子量 633 aa, 72 kDa
观测分子量 70 kDa
GenBank蛋白编号BC131713
基因名称 GBP6
Gene ID (NCBI) 163351
RRIDAB_2879195
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ6ZN66
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Guanylate-binding protein 6 (GBP6) is a member of the guanylate-binding protein family and is best known as a gene induced by interferon-γ signalling. Interferon-γ is produced predominantly by NK and T-cells, indicating that GBP6 is involved in host immune response (PMID: 28747659, 27029383). GBP6 has 2 isoforms with the molecular mass of 34 and 72 kDa.

实验方案

Product Specific Protocols
WB protocol for GBP6 antibody 23001-1-APDownload protocol
IHC protocol for GBP6 antibody 23001-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

PLoS One

Construction of ceRNA network to identify the lncRNA and mRNA related to non-small cell lung cancer.

Authors - Kui Xiao
View Article
humanWB

Clin Oral Investig

Guanylate-binding protein 6 is a novel biomarker for tumorigenesis and prognosis in tongue squamous cell carcinoma.

Authors - Pei-Feng Liu
View Article