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GBP6 Polyclonal antibody

GBP6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23001-1-AP

Print datasheet

Synonyms

Guanylate-binding protein 6, Guanine nucleotide-binding protein 6, GTP-binding protein 6, GBP-6, EC:3.6.5.-

验证数据展示

  • WB analysis of HeLa using 23001-1-AP

    WB analysis of HeLa using 23001-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23001-1-AP (GBP6 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 23001-1-AP

    IHC staining of human testis using 23001-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23001-1-AP (GBP6 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 23001-1-AP

    IHC staining of human testis using 23001-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23001-1-AP (GBP6 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human lung cancer using 23001-1-AP

    IHC staining of human lung cancer using 23001-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 23001-1-AP (GBP6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells
Positive IHC detected inhuman testis tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

23001-1-AP targets GBP6 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen GBP6 fusion protein Ag19196 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name guanylate binding protein family, member 6
Synonyms Guanylate-binding protein 6, Guanine nucleotide-binding protein 6, GTP-binding protein 6, GBP-6, EC:3.6.5.-
Calculated Molecular Weight 633 aa, 72 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC131713
Gene Symbol GBP6
Gene ID (NCBI) 163351
RRIDAB_2879195
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ6ZN66
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Guanylate-binding protein 6 (GBP6) is a member of the guanylate-binding protein family and is best known as a gene induced by interferon-γ signalling. Interferon-γ is produced predominantly by NK and T-cells, indicating that GBP6 is involved in host immune response (PMID: 28747659, 27029383). GBP6 has 2 isoforms with the molecular mass of 34 and 72 kDa.

实验方案

Product Specific Protocols
WB protocol for GBP6 antibody 23001-1-APDownload protocol
IHC protocol for GBP6 antibody 23001-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

PLoS One

Construction of ceRNA network to identify the lncRNA and mRNA related to non-small cell lung cancer.

Authors - Kui Xiao
View Article
humanWB

Clin Oral Investig

Guanylate-binding protein 6 is a novel biomarker for tumorigenesis and prognosis in tongue squamous cell carcinoma.

Authors - Pei-Feng Liu
View Article