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验证数据展示

  • WB analysis of Raji using 13447-1-AP

    WB analysis of Raji using 13447-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 13447-1-AP (GCET2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of Raji using 13447-1-AP

    IF Staining of Raji using 13447-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using GCET2 antibody (13447-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

GCET2 Polyclonal antibody

GCET2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 13447-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Germinal center-associated signaling and motility protein, Germinal center-associated lymphoma protein, Germinal center B-cell-expressed transcript 2 protein, GCSAM, GCAT2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRaji cells
Positive IF/ICC detected inRaji cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13447-1-AP targets GCET2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 GCET2 fusion protein Ag4280 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 germinal center expressed transcript 2
别名 Germinal center-associated signaling and motility protein, Germinal center-associated lymphoma protein, Germinal center B-cell-expressed transcript 2 protein, GCSAM, GCAT2
计算分子量 178 aa, 21 kDa
观测分子量 25 kDa
GenBank蛋白编号BC030506
基因名称 GCET2
Gene ID (NCBI) 257144
RRIDAB_2877949
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N6F7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GCET2, also named HGAL, is a newly gene that has been shown to be a useful marker for germinal centre (GC) B cells and GC B-cell derived malignancies, including follicular lymphomas and germinal centre B cell-like diffuse large B-cell lymphomas (GCB-DLBCLs), and is a useful prognosticator for DLBCLs. GCET2 may be an adaptor protein in GC B cells that transduces signals from GC B-cell membrane to the cytosol via its association with GRB2.

实验方案

Product Specific Protocols
WB protocol for GCET2 antibody 13447-1-APDownload protocol
IF protocol for GCET2 antibody 13447-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols