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GCK Monoclonal antibody, PBS Only

GCK Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C3A3

Cat no : 67216-1-PBS

Print datasheet

Synonyms

HK IV, Hexokinase-D, Hexokinase-4, Glucokinase, EC:2.7.1.1

验证数据展示

  • WB analysis using 67216-1-Ig (same clone as 67216-1-PBS)

    WB analysis using 67216-1-Ig (same clone as 67216-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.

  • WB analysis of pig liver using 67216-1-Ig (same clone as 67216-1-PBS)

    WB analysis of pig liver using 67216-1-Ig (same clone as 67216-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 67216-1-Ig (same clone as 67216-1-PBS)

    IF Staining of human liver cancer using 67216-1-Ig (same clone as 67216-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using GCK antibody (67216-1-Ig, Clone: 1C3A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 67216-1-Ig (same clone as 67216-1-PBS)

    IF Staining of human liver cancer using 67216-1-Ig (same clone as 67216-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using GCK antibody (67216-1-Ig, Clone: 1C3A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67216-1-Ig (same clone as 67216-1-PBS)

    IHC staining of human liver cancer using 67216-1-Ig (same clone as 67216-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67216-1-Ig (GCK antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GCK antibody included
  • IHC staining of human liver cancer using 67216-1-Ig (same clone as 67216-1-PBS)

    IHC staining of human liver cancer using 67216-1-Ig (same clone as 67216-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67216-1-Ig (GCK antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GCK antibody included
Planning an IHC experiment? We recommend our IHCeasy GCK Ready-To-Use IHC Kit. GCK primary antibody included.

产品信息

67216-1-PBS targets GCK in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen GCK fusion protein Ag8116 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name glucokinase (hexokinase 4)
Synonyms HK IV, Hexokinase-D, Hexokinase-4, Glucokinase, EC:2.7.1.1
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC001890
Gene Symbol GCK
Gene ID (NCBI) 2645
RRIDAB_2882508
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP35557
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Glucokinase (GCK) is a structurally and functionally unique member of hexokinase family. It is expressed only in mammalian liver and pancreatic islet beta cells. Because of its unique functional characteristics, the enzyme plays an important regulatory role in glucose metabolism.The rate of glucose metabolism in liver and pancreas is a function of the activity of the enzyme(PMID:1740341). Moreover, GCK has been found to have relationship with diabetes. Defects in GCK are the cause of maturity-onset diabetes of the young type 2 (MODY2) and familial hyperinsulinemic hypoglycemia type 3 (HHF3). It has 3 isoforms produced by alternative splicing with the same molecular mass of 52 kDa.