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验证数据展示

  • IHC staining of human placenta using 21724-1-AP

    IHC staining of human placenta using 21724-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21724-1-AP (GCM1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 21724-1-AP

    IHC staining of human placenta using 21724-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21724-1-AP (GCM1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HepG2 using 21724-1-AP

    WB analysis of HepG2 using 21724-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21724-1-AP (GCM1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

GCM1 Polyclonal antibody

GCM1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21724-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

hGCMa, Glial cells missing homolog 1, GCMA, GCM motif protein 1, Chorion-specific transcription factor GCMa

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

21724-1-AP targets GCM1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 GCM1 fusion protein Ag16383 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glial cells missing homolog 1 (Drosophila)
别名 hGCMa, Glial cells missing homolog 1, GCMA, GCM motif protein 1, Chorion-specific transcription factor GCMa
计算分子量 436 aa, 49 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC096288
基因名称 GCM1
Gene ID (NCBI) 8521
RRIDAB_2878913
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NP62
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GCM1 antibody 21724-1-APDownload protocol
IHC protocol for GCM1 antibody 21724-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Reprod Biol Endocrinol

Alpha-actinin-4 is essential for maintaining normal trophoblast proliferation and differentiation during early pregnancy.

Authors - Wei Peng
View Article
humanWB

BMC Med

Epigenetic drug screening for trophoblast syncytialization reveals a novel role for MLL1 in regulating fetoplacental growth

Authors - Jiayi Wu
View Article