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GCM1 Polyclonal antibody

GCM1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21724-1-AP

Print datasheet

Synonyms

hGCMa, Glial cells missing homolog 1, GCMA, GCM motif protein 1, Chorion-specific transcription factor GCMa

验证数据展示

  • IHC staining of human placenta using 21724-1-AP

    IHC staining of human placenta using 21724-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21724-1-AP (GCM1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 21724-1-AP

    IHC staining of human placenta using 21724-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21724-1-AP (GCM1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HepG2 using 21724-1-AP

    WB analysis of HepG2 using 21724-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21724-1-AP (GCM1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

21724-1-AP targets GCM1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen GCM1 fusion protein Ag16383 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glial cells missing homolog 1 (Drosophila)
Synonyms hGCMa, Glial cells missing homolog 1, GCMA, GCM motif protein 1, Chorion-specific transcription factor GCMa
Calculated Molecular Weight 436 aa, 49 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC096288
Gene Symbol GCM1
Gene ID (NCBI) 8521
RRIDAB_2878913
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NP62
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GCM1 antibody 21724-1-APDownload protocol
IHC protocol for GCM1 antibody 21724-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Reprod Biol Endocrinol

Alpha-actinin-4 is essential for maintaining normal trophoblast proliferation and differentiation during early pregnancy.

Authors - Wei Peng
View Article
humanWB

BMC Med

Epigenetic drug screening for trophoblast syncytialization reveals a novel role for MLL1 in regulating fetoplacental growth

Authors - Jiayi Wu
View Article