GCNA Polyclonal antibody

GCNA Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25516-1-AP

Print datasheet

Synonyms

acidic repeat containing, ACRC, GCNA, NAAR1



经过测试的应用

Positive WB detected inhuman testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25516-1-AP targets GCNA in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen GCNA fusion protein Ag21895 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name acidic repeat containing
Synonyms acidic repeat containing, ACRC, GCNA, NAAR1
Calculated Molecular Weight 691 aa, 76 kDa
Observed Molecular Weight90 kDa
GenBank Accession NumberBC136259
Gene Symbol GCNA
Gene ID (NCBI) 93953
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96QF7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GCNA, also known as ACRC, belongs to the serine-aspartate repeat-containing protein (SDr) family. GCNA has remained tightly associated with sexual reproduction showing enriched expression within germ cells in both invertebrate and vertebrate species. GCNA has recently been implicated in resolving DPCs in germ cells and early embryos. GCNA is targeted to SUMOylated DPC proteins through its SUMO-interacting motifs (SIMs), reinforcing the importance of SUMOylation in DPC repair. The amino acid sequence homology of GCNA in humans and mice is very low. The calculated molecular weight of GCNA is 76 kDa. Sometimes higher molecular weight around 90 kDa can also be observed, which may be caused by SUMO modification (PMID: 31839537, 32329115).

实验方案

Product Specific Protocols
WB protocol for GCNA antibody 25516-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols