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验证数据展示

  • WB analysis using 84124-4-RR

    WB analysis using 84124-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84124-4-RR (GCNA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of LNCaP using 84124-4-RR

    IF Staining of LNCaP using 84124-4-RR

    Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using GCNA antibody (84124-4-RR, Clone: 241428A9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84124-4-RR

    Affinity and Kinetic Characterization of 84124-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84124-4-RR against Human GCNA were performed. The affinity constant is below 1 pM.

GCNA Recombinant antibody

GCNA Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84124-4-RR

产品说明书

CloneNo. 241428A9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

ACRC, Germ cell nuclear antigen, Germ cell nuclear acidic protein, Germ cell nuclear acidic peptidase, Acidic repeat-containing protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, DU 145 cells, MCF-7 cells, A549 cells, HepG2 cells, PC-3 cells, human testis tissue
Positive IF/ICC detected inLNCaP cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84124-4-RR targets GCNA in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 GCNA fusion protein Ag21895 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 acidic repeat containing
别名 ACRC, Germ cell nuclear antigen, Germ cell nuclear acidic protein, Germ cell nuclear acidic peptidase, Acidic repeat-containing protein
计算分子量 691 aa, 76 kDa
观测分子量90 kDa
GenBank蛋白编号BC136259
基因名称 GCNA
Gene ID (NCBI) 93953
RRIDAB_3671685
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ96QF7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GCNA, also known as ACRC, belongs to the serine-aspartate repeat-containing protein (SDr) family. GCNA has remained tightly associated with sexual reproduction showing enriched expression within germ cells in both invertebrate and vertebrate species. GCNA has recently been implicated in resolving DPCs in germ cells and early embryos. GCNA is targeted to SUMOylated DPC proteins through its SUMO-interacting motifs (SIMs), reinforcing the importance of SUMOylation in DPC repair. The amino acid sequence homology of GCNA in humans and mice is very low. The calculated molecular weight of GCNA is 76 kDa. Sometimes higher molecular weight around 90 kDa can also be observed, which may be caused by SUMO modification (PMID: 31839537, 32329115).

实验方案

Product Specific Protocols
WB protocol for GCNA antibody 84124-4-RRDownload protocol
IF protocol for GCNA antibody 84124-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols