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GDF-15 Polyclonal antibody

GDF-15 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27455-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA and More (1)

GDF15, GDF 15, growth differentiation factor 15, Growth/differentiation factor 15, MIC-1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHepG2 cells, HT-1080 cells, LNCaP cells, human placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27455-1-AP targets GDF-15 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human
免疫原 GDF-15 fusion protein Ag26760 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 growth differentiation factor 15
别名 GDF15, GDF 15, growth differentiation factor 15, Growth/differentiation factor 15, MIC-1
计算分子量 34 kDa
观测分子量 34 kDa
GenBank蛋白编号BC008962
基因名称 GDF15
Gene ID (NCBI) 9518
RRIDAB_2880875
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99988
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Growth differentiation factor 15 (GDF15), also known as macrophage inhibitory cytokine-1 (MIC-1), is a protein of the transforming growth factor beta (TGFb) superfamily that regulates inflammatory and apoptotic pathways in injured tissues and during disease processes. GDF15 is a stress-induced cytokine and associated with hypoxia, inflammation and oxidative stress, it is also released from endothelial cells after stimulation with pro-inflammatory cytokines. GDF15 has been suggested as a target and biomarker for cardiovascular disease as it plays a cardioprotective role in the adult heart. GDF15 is highly expressed in the placenta. GDF15 is known to be involved in human embryo development and necessary for the maintenance of pregnancy. GDF15 is also produced by adipocytes in response to oxidative stress, a well-known factor associated with preeclampsia.

实验方案

Product Specific Protocols
WB protocol for GDF-15 antibody 27455-1-APDownload protocol
IHC protocol for GDF-15 antibody 27455-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Dual functions of SPOP and ERG dictate androgen therapy responses in prostate cancer.

Authors - Tiziano Bernasocchi
humanWB

Cancers (Basel)

GDF15 Is an Eribulin Response Biomarker also Required for Survival of DTP Breast Cancer Cells.

Authors - Chiara Bellio
  • KD Validated
humanWB

J Cell Physiol

Tumor-Derived GDF15 Induces Tumor Associated Fibroblast Transformation From BMSCs and Fibroblasts in Oral Squamous Cell Carcinoma

Authors - Jingjing Zhao
  • KD Validated
humanWB

Mol Neurobiol

Exosomes Derived from Meningitic Escherichia coli-Infected Brain Microvascular Endothelial Cells Facilitate Astrocyte Activation

Authors - Ruicheng Yang
humanWB

Antioxidants (Basel)

Grape Seed Proanthocyanidins Induce Apoptosis and Cell Cycle Arrest of HepG2 Cells Accompanied by Induction of the MAPK Pathway and NAG-1.

Authors - Lihua Wang
humanWB

Int J Biol Sci

Long noncoding RNA SH3PXD2A-AS1 promotes colorectal cancer progression by regulating p53-mediated gene transcription.

Authors - Pingfu Hou
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