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GDF-15 Polyclonal antibody

GDF-15 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27845-1-AP

Print datasheet

Synonyms

GDF15, GDF 15, NAG-1, MIC-1, Growth/differentiation factor 15

验证数据展示

  • WB analysis using 27845-1-AP

    WB analysis using 27845-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27845-1-AP (GDF15 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 27845-1-AP

    IHC staining of human placenta using 27845-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27845-1-AP (GDF15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 27845-1-AP

    IHC staining of human placenta using 27845-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27845-1-AP (GDF15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHT-1080 cells, HepG2 cells, LNCaP cells, human placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27845-1-AP targets GDF-15 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen GDF-15 fusion protein Ag24090 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name growth differentiation factor 15
Synonyms GDF15, GDF 15, NAG-1, MIC-1, Growth/differentiation factor 15
Calculated Molecular Weight 34 kDa
Observed Molecular Weight34 kDa
GenBank Accession NumberBC008962
Gene Symbol GDF15
Gene ID (NCBI) 9518
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99988
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Growth differentiation factor 15 (GDF15), also known as macrophage inhibitory cytokine-1 (MIC-1), is a protein of the transforming growth factor beta (TGFb) superfamily that regulates inflammatory and apoptotic pathways in injured tissues and during disease processes. GDF15 is a stress-induced cytokine and associated with hypoxia, inflammation and oxidative stress, it is also released from endothelial cells after stimulation with pro-inflammatory cytokines. GDF15 has been suggested as a target and biomarker for cardiovascular disease as it plays a cardioprotective role in the adult heart. GDF15 is highly expressed in the placenta. GDF15 is known to be involved in human embryo development and necessary for the maintenance of pregnancy. GDF15 is also produced by adipocytes in response to oxidative stress, a well-known factor associated with preeclampsia.

实验方案

Product Specific Protocols
WB protocol for GDF-15 antibody 27845-1-APDownload protocol
IHC protocol for GDF-15 antibody 27845-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols