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验证数据展示

  • WB analysis using 29309-1-AP

    WB analysis using 29309-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29309-1-AP (GDF9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse ovary using 29309-1-AP

    IHC staining of mouse ovary using 29309-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 29309-1-AP (GDF9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GDF9 antibody included
  • IF Staining of SKOV-3 using 29309-1-AP

    IF Staining of SKOV-3 using 29309-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using GDF9 antibody (29309-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

GDF9 Polyclonal antibody

GDF9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 29309-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

GDF-9, GDF 9

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inA2780 cells, SKOV-3 cells, mouse ovary tissue, rat ovary tissue
Positive IHC detected inmouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSKOV-3 cells
Planning an IHC experiment? We recommend our IHCeasy GDF9 Ready-To-Use IHC Kit. GDF9 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29309-1-AP targets GDF9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen GDF9 fusion protein Ag29603 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name growth differentiation factor 9
Synonyms GDF-9, GDF 9
Calculated Molecular Weight 454 aa, 51 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC096228
Gene Symbol GDF9
Gene ID (NCBI) 2661
RRIDAB_3086112
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60383
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GDF9 (Growth/differentiation factor 9) belongs to the TGF-beta family. GDF9 is required for ovarian folliculogenesis. GDF9 and BMP15 are oocyte-secreted factors with a leading role in the control of ovarian function in female reproduction, modulating both the cell fate of the somatic granulosa cells and the quality and developmental competence of the egg (PMID: 29544636). GDF9 promotes cell transition from G0/G1 to S and G2/M phases, through an increase of CCND1 and CCNE1 expression, and RB1 phosphorylation (PMID: 19366876). It regulates STAR expression and cAMP-dependent progesterone release in granulosa and thecal cells. GDF9 attenuates the suppressive effects of activin A on STAR expression and progesterone production by increasing the expression of inhibin B (PMID: 21829661). It suppresses FST and FSTL3 production in granulosa-lutein cells  (PMID: 21632818, PMID: 21829661).

实验方案

Product Specific Protocols
WB protocol for GDF9 antibody 29309-1-APDownload protocol
IHC protocol for GDF9 antibody 29309-1-APDownload protocol
IF protocol for GDF9 antibody 29309-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols