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验证数据展示

  • IHC staining of human skin using 55096-1-AP

    IHC staining of human skin using 55096-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 55096-1-AP (GDI2 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with GDI2 antibody included
  • IHC staining of human skin using 55096-1-AP

    IHC staining of human skin using 55096-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 55096-1-AP (GDI2 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with GDI2 antibody included

GDI2 Polyclonal antibody

GDI2 Polyclonal Antibody for IHC, ELISA
Cat No. 55096-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, ELISA

别名

GDI 2, GDI2, GDP dissociation inhibitor 2, Rab GDI beta, RABGDIB

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman skin tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy GDI2 Ready-To-Use IHC Kit. GDI2 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55096-1-AP targets GDI2 in IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GDP dissociation inhibitor 2
别名 GDI 2, GDI2, GDP dissociation inhibitor 2, Rab GDI beta, RABGDIB
计算分子量 51 kDa
GenBank蛋白编号NM_001494
基因名称 GDI2
Gene ID (NCBI) 2665
RRIDAB_11183032
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP50395
储存缓冲液 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI2 distributes ubiquitously, displaying a membrane bound location in perinuclear regions of cells. GDI-2 was thought to be involved in cellular response to insulin. It electrophoreses as a 46kd protein in SDS-PAGE. (PMID: 7929030; PMID: 19570034). The antibody is specific to GDI2. And it has no cross reaction to GDI1.

实验方案

Product Specific Protocols
IHC protocol for GDI2 antibody 55096-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols