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GDI2 Polyclonal antibody

GDI2 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 55096-1-AP

Print datasheet

Synonyms

GDI 2, GDI2, GDP dissociation inhibitor 2, Rab GDI beta, RABGDIB

验证数据展示

  • IHC staining of human skin using 55096-1-AP

    IHC staining of human skin using 55096-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 55096-1-AP (GDI2 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with GDI2 antibody included
  • IHC staining of human skin using 55096-1-AP

    IHC staining of human skin using 55096-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 55096-1-AP (GDI2 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with GDI2 antibody included

经过测试的应用

Positive IHC detected inhuman skin tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy GDI2 Ready-To-Use IHC Kit. GDI2 primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55096-1-AP targets GDI2 in IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GDP dissociation inhibitor 2
Synonyms GDI 2, GDI2, GDP dissociation inhibitor 2, Rab GDI beta, RABGDIB
Calculated Molecular Weight 51 kDa
GenBank Accession NumberNM_001494
Gene Symbol GDI2
Gene ID (NCBI) 2665
RRIDAB_11183032
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP50395
Storage Buffer 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI2 distributes ubiquitously, displaying a membrane bound location in perinuclear regions of cells. GDI-2 was thought to be involved in cellular response to insulin. It electrophoreses as a 46kd protein in SDS-PAGE. (PMID: 7929030; PMID: 19570034). The antibody is specific to GDI2. And it has no cross reaction to GDI1.

实验方案

Product Specific Protocols
IHC protocol for GDI2 antibody 55096-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols