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GDI2 Monoclonal antibody, PBS Only

GDI2 Monoclonal Antibody for WB,IHC,IF,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB,IHC,IF,ELISA

Conjugate

Unconjugated

CloneNo.

4B8F3

Cat No : 60078-1-PBS

Print datasheet

Synonyms

FLJ16452, FLJ37352, GDI 2, GDI2, GDP dissociation inhibitor 2, Rab GDI beta, RABGDIB

验证数据展示

  • WB analysis using 60078-1-Ig (same clone as 60078-1-PBS)

    WB analysis using 60078-1-Ig (same clone as 60078-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60078-1-Ig (GDI2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60078-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 60078-1-Ig (same clone as 60078-1-PBS)

    IHC staining of human breast cancer using 60078-1-Ig (same clone as 60078-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60078-1-Ig (GDI2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60078-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GDI2 antibody included
  • IHC staining of human breast cancer using 60078-1-Ig (same clone as 60078-1-PBS)

    IHC staining of human breast cancer using 60078-1-Ig (same clone as 60078-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60078-1-Ig (GDI2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60078-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GDI2 antibody included
  • IF Staining of HeLa using 60078-1-Ig (same clone as 60078-1-PBS)

    IF Staining of HeLa using 60078-1-Ig (same clone as 60078-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 60078-1-Ig (GDI2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60078-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 60078-1-Ig (same clone as 60078-1-PBS)

    IF Staining of HeLa using 60078-1-Ig (same clone as 60078-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GDI2 antibody (60078-1-Ig, Clone: 4B8F3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60078-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy GDI2 Ready-To-Use IHC Kit. GDI2 primary antibody included.

产品信息

60078-1-PBS targets GDI2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB,IHC,IF,ELISA Application Description
Tested Reactivity human, mouse
Immunogen GDI2 fusion protein Ag4613 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name GDP dissociation inhibitor 2
Synonyms FLJ16452, FLJ37352, GDI 2, GDI2, GDP dissociation inhibitor 2, Rab GDI beta, RABGDIB
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC005145
Gene Symbol GDI2
Gene ID (NCBI) 2665
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP50395
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

背景介绍

GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI2 distributes ubiquitously, displaying a membrane bound location in perinuclear regions of cells. GDI-2 was thought to be involved in cellular response to insulin. It electrophoreses as a 46kd protein in SDS-PAGE. (PMID: 7929030; PMID: 19570034). This antibody can bind both GDIs for the close sequences.