• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 60078-1-Ig (same clone as 60078-1-PBS)

    WB analysis using 60078-1-Ig (same clone as 60078-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60078-1-Ig (GDI2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60078-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 60078-1-Ig (same clone as 60078-1-PBS)

    IHC staining of human breast cancer using 60078-1-Ig (same clone as 60078-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60078-1-Ig (GDI2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60078-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GDI2 antibody included
  • IHC staining of human breast cancer using 60078-1-Ig (same clone as 60078-1-PBS)

    IHC staining of human breast cancer using 60078-1-Ig (same clone as 60078-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60078-1-Ig (GDI2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60078-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GDI2 antibody included
  • IF Staining of HeLa using 60078-1-Ig (same clone as 60078-1-PBS)

    IF Staining of HeLa using 60078-1-Ig (same clone as 60078-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 60078-1-Ig (GDI2 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60078-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 60078-1-Ig (same clone as 60078-1-PBS)

    IF Staining of HeLa using 60078-1-Ig (same clone as 60078-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GDI2 antibody (60078-1-Ig, Clone: 4B8F3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60078-1-PBS in a different storage buffer formulation.

GDI2 Monoclonal antibody, PBS Only

GDI2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 60078-1-PBS

产品说明书

CloneNo. 4B8F3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

FLJ16452, FLJ37352, GDI 2, GDI2, GDP dissociation inhibitor 2, Rab GDI beta, RABGDIB

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy GDI2 Ready-To-Use IHC Kit. GDI2 primary antibody included.

产品信息

60078-1-PBS targets GDI2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 GDI2 fusion protein Ag4613 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 GDP dissociation inhibitor 2
别名 FLJ16452, FLJ37352, GDI 2, GDI2, GDP dissociation inhibitor 2, Rab GDI beta, RABGDIB
计算分子量 47 kDa
观测分子量 46 kDa
GenBank蛋白编号BC005145
基因名称 GDI2
Gene ID (NCBI) 2665
RRIDAB_2232457
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP50395
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI2 distributes ubiquitously, displaying a membrane bound location in perinuclear regions of cells. GDI-2 was thought to be involved in cellular response to insulin. It electrophoreses as a 46kd protein in SDS-PAGE. (PMID: 7929030; PMID: 19570034). This antibody can bind both GDIs for the close sequences.