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验证数据展示

  • WB analysis using 26179-1-AP

    WB analysis using 26179-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26179-1-AP (GDNF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 26179-1-AP

    IHC staining of mouse brain using 26179-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26179-1-AP (GDNF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 26179-1-AP

    IHC staining of mouse brain using 26179-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26179-1-AP (GDNF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of SH-SY5Y using 26179-1-AP

    IF Staining of SH-SY5Y using 26179-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using GDNF antibody (26179-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

GDNF Polyclonal antibody

GDNF Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 26179-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

ATF, ATF1, ATF2, GDNF, HFB1 GDNF, hGDNF

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inDU 145 cells, HEK-293 cells, PC-3 cells, U-251 cells, U-87 MG cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26179-1-AP targets GDNF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 GDNF fusion protein Ag24068 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glial cell derived neurotrophic factor
别名 ATF, ATF1, ATF2, GDNF, HFB1 GDNF, hGDNF
计算分子量 211 aa, 24 kDa
观测分子量 32-40 kDa
GenBank蛋白编号BC069369
基因名称 GDNF
Gene ID (NCBI) 2668
RRIDAB_3085846
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP39905
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glial-derived neurotrophic factor (GDNF) is a member of the transforming growth factor (TGF)-α family and exhibits potent neuroprotective activity. GDNF is a potent promoter of neuronal survival in the CNS and peripheral nervous system (PNS). It has a relatively high speciicity for dopaminergic neurons and thus become an efective treatment for Parkinson's diseases (PD), with clinical trials currently in progress. Protein dimers of GDNF have a reported molecular weight of 32-40 kDa(PMID: 17982417, PMID: 23251488, PMID: 11429269).

实验方案

Product Specific Protocols
WB protocol for GDNF antibody 26179-1-APDownload protocol
IHC protocol for GDNF antibody 26179-1-APDownload protocol
IF protocol for GDNF antibody 26179-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols