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验证数据展示

  • IHC staining of human placenta using 25703-1-AP

    IHC staining of human placenta using 25703-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25703-1-AP (GDPD5 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 25703-1-AP

    IHC staining of human placenta using 25703-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25703-1-AP (GDPD5 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of K-562 using 25703-1-AP

    WB analysis of K-562 using 25703-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 25703-1-AP (GDPD5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

GDPD5 Polyclonal antibody

GDPD5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25703-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA and More (1)

别名

Phosphoinositide phospholipase C GDPD5, Glycerophosphodiester phosphodiesterase domain-containing protein 5, Glycerophosphocholine phosphodiesterase GDPD5, GDE2, EC:3.1.4.2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, COLO 320 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25703-1-AP targets GDPD5 in WB, IHC, CoIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, CoIP
经测试反应性 human
文献引用反应性human
免疫原 GDPD5 fusion protein Ag22530 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glycerophosphodiester phosphodiesterase domain containing 5
别名 Phosphoinositide phospholipase C GDPD5, Glycerophosphodiester phosphodiesterase domain-containing protein 5, Glycerophosphocholine phosphodiesterase GDPD5, GDE2, EC:3.1.4.2
计算分子量 605 aa, 69 kDa
观测分子量 51 kDa
GenBank蛋白编号BC018771
基因名称 GDPD5
Gene ID (NCBI) 81544
RRIDAB_2880200
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WTR4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GDPD5(Glycerophosphodiester phosphodiesterase domain-containing protein 5) is also named as GDE2 and belongs to the glycerophosphoryl diester phosphodiesterase family. The deduced sequence of GDE2 contains 607 amino acids with seven putative transmembrane regions. GDPD5 is a glycerophosphocholine phosphodiesterase that osmotically regulates the osmoprotective organic osmolyte GPC(PMID:18667693). It has 5 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for GDPD5 antibody 25703-1-APDownload protocol
IHC protocol for GDPD5 antibody 25703-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,CoIP

Cancer Res

PRKDC Induces Chemoresistance in Osteosarcoma by Recruiting GDE2 to Stabilize GNAS and Activate AKT

Authors - Wenchao Zhang
  • KD Validated
View Article
humanWB

J Biol Chem

Persistent ER stress causes GPI anchor deficit to convert a GPI-anchored prion protein (PrP) into pro-PrP via the ATF6-miR449c-5p-PIGV axis

Authors - JingFeng Li
View Article