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GDPD5 Polyclonal antibody

GDPD5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat No : 25703-1-AP

Print datasheet

Synonyms

GDE2, GDPD5, PP1665, PP9363, UNQ1850/PRO3580

验证数据展示

  • IHC staining of human placenta using 25703-1-AP

    IHC staining of human placenta using 25703-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25703-1-AP (GDPD5 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 25703-1-AP

    IHC staining of human placenta using 25703-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25703-1-AP (GDPD5 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of K-562 using 25703-1-AP

    WB analysis of K-562 using 25703-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 25703-1-AP (GDPD5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inK-562 cells, COLO 320 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 25703-1-AP is GDPD5 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen GDPD5 fusion protein Ag22530 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glycerophosphodiester phosphodiesterase domain containing 5
Synonyms GDE2, GDPD5, PP1665, PP9363, UNQ1850/PRO3580
Calculated Molecular Weight 605 aa, 69 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC018771
Gene Symbol GDPD5
Gene ID (NCBI) 81544
RRIDAB_2880200
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WTR4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GDPD5(Glycerophosphodiester phosphodiesterase domain-containing protein 5) is also named as GDE2 and belongs to the glycerophosphoryl diester phosphodiesterase family. The deduced sequence of GDE2 contains 607 amino acids with seven putative transmembrane regions. GDPD5 is a glycerophosphocholine phosphodiesterase that osmotically regulates the osmoprotective organic osmolyte GPC(PMID:18667693). It has 5 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for GDPD5 antibody 25703-1-APDownload protocol
IHC protocol for GDPD5 antibody 25703-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Biol Chem

Persistent ER stress causes GPI anchor deficit to convert a GPI-anchored prion protein (PrP) into pro-PrP via the ATF6-miR449c-5p-PIGV axis

Authors - JingFeng Li
View Article