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验证数据展示

  • WB analysis of human brain using 17028-1-AP

    WB analysis of human brain using 17028-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17028-1-AP (GEFT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 17028-1-AP

    IP experiment of HEK-293 using 17028-1-AP

    IP result of anti-GEFT (IP:17028-1-AP, 4ug; Detection:17028-1-AP 1:400) with HEK-293 cells lysate 2000ug.

GEFT Polyclonal antibody

GEFT Polyclonal Antibody for WB, IP, ELISA
Cat No. 17028-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

ARHGEF25, GEFT, p63RhoGEF, RhoA/RAC/CDC42 exchange factor

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman brain tissue
Positive IP detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17028-1-AP targets GEFT in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GEFT fusion protein Ag10436 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RhoA/RAC/CDC42 exchange factor
别名 ARHGEF25, GEFT, p63RhoGEF, RhoA/RAC/CDC42 exchange factor
计算分子量64 kDa
观测分子量 64 kDa
GenBank蛋白编号BC012860
基因名称 GEFT
Gene ID (NCBI) 115557
RRIDAB_2279083
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86VW2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GEFT, also named as p63RhoGEF, may play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. GETF links specifically G alpha q/11-coupled receptors to RHOA activation. GEFT may be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. It is involved in skeletal myogenesis. GEFT is expressed as three isoforms (64, 53 and 68 kDa).

实验方案

Product Specific Protocols
WB protocol for GEFT antibody 17028-1-APDownload protocol
IP protocol for GEFT antibody 17028-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols