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GEMIN8 Monoclonal antibody, PBS Only

GEMIN8 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A6B4

Cat no : 67871-1-PBS

Print datasheet

Synonyms

FAM51A1, FLJ20514, FLJ43072, Gem associated protein 8, Gemin 8, GEMIN8, Protein FAM51A1

验证数据展示

  • WB analysis of HeLa using 67871-1-Ig (same clone as 67871-1-PBS)

    WB analysis of HeLa using 67871-1-Ig (same clone as 67871-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67871-1-Ig (GEMIN8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67871-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 67871-1-Ig (same clone as 67871-1-PBS)

    WB analysis of NIH/3T3 using 67871-1-Ig (same clone as 67871-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67871-1-Ig (GEMIN8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67871-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67871-1-Ig (same clone as 67871-1-PBS)

    IF Staining of HepG2 using 67871-1-Ig (same clone as 67871-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GEMIN8 antibody (67871-1-Ig, Clone: 1A6B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). DAPI (Blue). This data was developed using the same antibody clone with 67871-1-PBS in a different storage buffer formulation.

产品信息

67871-1-PBS targets GEMIN8 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen GEMIN8 fusion protein Ag6774 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name gem (nuclear organelle) associated protein 8
Synonyms FAM51A1, FLJ20514, FLJ43072, Gem associated protein 8, Gemin 8, GEMIN8, Protein FAM51A1
Calculated Molecular Weight 29 kDa
GenBank Accession NumberBC003607
Gene Symbol GEMIN8
Gene ID (NCBI) 54960
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9NWZ8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GEMIN8, also named as FAM51A1 and Gemin-8, is associated with the SMN complex and is localized in the cytoplasm and in the nucleus, where it is highly concentrated in Gems. Gemin6, Gemin7, and Unrip form a stable cytoplasmic complex whose association with SMN requires Gemin8. Gemin8 binds directly to SMN and mediates its interaction with the Gemin6/Gemin7 heterodimer. This antibody recognize GEMIN8(28-32kd) only.