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验证数据展示

  • WB analysis of HeLa using 67871-1-Ig (same clone as 67871-1-PBS)

    WB analysis of HeLa using 67871-1-Ig (same clone as 67871-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67871-1-Ig (GEMIN8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67871-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 67871-1-Ig (same clone as 67871-1-PBS)

    WB analysis of NIH/3T3 using 67871-1-Ig (same clone as 67871-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67871-1-Ig (GEMIN8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67871-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67871-1-Ig (same clone as 67871-1-PBS)

    IF Staining of HepG2 using 67871-1-Ig (same clone as 67871-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GEMIN8 antibody (67871-1-Ig, Clone: 1A6B4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). DAPI (Blue). This data was developed using the same antibody clone with 67871-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67871-1-Ig (same clone as 67871-1-PBS)

    IHC staining of mouse brain using 67871-1-Ig (same clone as 67871-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67871-1-Ig (GEMIN8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67871-1-PBS in a different storage buffer formulation.

GEMIN8 Monoclonal antibody, PBS Only

GEMIN8 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67871-1-PBS

产品说明书

CloneNo. 1A6B4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Gemin-8, Gemin 8, Gem-associated protein 8, FAM51A1, 1A6B4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67871-1-PBS targets GEMIN8 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GEMIN8 fusion protein Ag6774 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 gem (nuclear organelle) associated protein 8
别名 Gemin-8, Gemin 8, Gem-associated protein 8, FAM51A1, 1A6B4
计算分子量 29 kDa
GenBank蛋白编号BC003607
基因名称 GEMIN8
Gene ID (NCBI) 54960
RRIDAB_2918629
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9NWZ8
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GEMIN8, also named as FAM51A1 and Gemin-8, is associated with the SMN complex and is localized in the cytoplasm and in the nucleus, where it is highly concentrated in Gems. Gemin6, Gemin7, and Unrip form a stable cytoplasmic complex whose association with SMN requires Gemin8. Gemin8 binds directly to SMN and mediates its interaction with the Gemin6/Gemin7 heterodimer. This antibody recognize GEMIN8(28-32kd) only.