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验证数据展示

  • WB analysis using 23935-1-AP (same clone as 23935-1-PBS)

    WB analysis using 23935-1-AP (same clone as 23935-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23935-1-AP (GFAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23935-1-PBS in a different storage buffer formulation.

  • WB analysis of U-251 using 23935-1-AP (same clone as 23935-1-PBS)

    WB analysis of U-251 using 23935-1-AP (same clone as 23935-1-PBS)

    WB result of GFAP antibody (23935-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GFAP transfected U-251 cells. This data was developed using the same antibody clone with 23935-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 23935-1-AP (same clone as 23935-1-PBS)

    IP experiment of mouse brain using 23935-1-AP (same clone as 23935-1-PBS)

    IP result of anti-GFAP (IP:23935-1-AP, 4ug; Detection:23935-1-AP 1:20000) with mouse brain tissue lysate 1280 ug. This data was developed using the same antibody clone with 23935-1-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 23935-1-AP (same clone as 23935-1-PBS)

    IF Staining of rat brain using 23935-1-AP (same clone as 23935-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using GFAP antibody (23935-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23935-1-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 23935-1-AP (same clone as 23935-1-PBS)

    IF Staining of rat brain using 23935-1-AP (same clone as 23935-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using GFAP antibody (23935-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23935-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 23935-1-AP (same clone as 23935-1-PBS)

    IHC staining of mouse brain using 23935-1-AP (same clone as 23935-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23935-1-AP (GFAP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23935-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GFAP antibody included
  • IHC staining of mouse brain using 23935-1-AP (same clone as 23935-1-PBS)

    IHC staining of mouse brain using 23935-1-AP (same clone as 23935-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23935-1-AP (GFAP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23935-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GFAP antibody included
  • IF Staining of mouse brain using 23935-1-AP (same clone as 23935-1-PBS)

    IF Staining of mouse brain using 23935-1-AP (same clone as 23935-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using GFAP antibody (23935-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 23935-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

GFAP Polyclonal antibody, PBS Only

GFAP Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA
Cat No. 23935-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA

别名

glial fibrillary acidic protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy GFAP Ready-To-Use IHC Kit. GFAP primary antibody included.

产品信息

23935-1-PBS targets GFAP in WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GFAP fusion protein Ag20853 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glial fibrillary acidic protein
别名 glial fibrillary acidic protein
计算分子量 432 aa, 50 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC013596
基因名称 GFAP
Gene ID (NCBI) 2670
RRIDAB_2879367
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP14136
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Function

GFAP (Glial fibrillary acidic protein) is a type III intermediate filament (IF) protein specific to the central  nervous system (CNS). GFAP is one of the  main components of the intermediate filament network in astrocytes and has been proposed as playing a role in cell migration, cell motility, maintaining mechanical strength, and in mitosis.

Tissue specificity

GFAP is expressed in central nervous system cells, predominantly in astrocytes. GFAP is commonly used as an astrocyte marker.  However, GFAP is also present in peripheral glia and in non-CNS cells, including fibroblasts, chondrocytes, lymphocytes, and liver stellate cells (PMID: 21219963).

Involvement in disease

  • Mutations in GFAP lead to Alexander disease (OMIM: 203450), an autosomal dominant CNS disorder. The mutations  present in affected individuals are thought to be gain-of-function. 

  • Upregulation of GFAP is a hallmark of reactive astrocytes, in which GFAP is present in hypertrophic cellular  processes. Reactive astrogliosis is present in many neurological disorders, such as stroke, various  neurodegenerative diseases (including Alzheimer's and Parkinson's disease), and neurotrauma. 

Isoforms

Astrocytes express 10 different isoforms of GFAP that differ in the rod and tail domains (PMID: 25726916),  which means that they differ in molecular size. Isoform expression varies during the development and across different subtypes of astrocytes. Not all isoforms are upregulated in reactive astrocytes.

Post-translational modifications

Intermediate filament proteins are regulated by phosphorylation. Six phosphorylation sites have been identified in GFAP protein, at least some of which are reported to control filament assembly (PMID: 21219963).

Cellular localization

GFAP localizes to intermediate filaments and stains well in astrocyte cellular processes.