验证数据展示
经过测试的应用
Positive WB detected in | K-562 cells, HL-60 cells, mouse thymus tissue, PC-3 cells, Raji cells, RAW264.7 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IHC | See 1 publications below |
产品信息
14198-1-AP targets GFI1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, ELISA Application Description |
Cited Applications | IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | mouse |
Immunogen | GFI1 fusion protein Ag5401 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | growth factor independent 1 transcription repressor |
Synonyms | GFI 1, GFI1, Zinc finger protein 163, Zinc finger protein Gfi 1, ZNF163 |
Calculated Molecular Weight | 45 kDa |
Observed Molecular Weight | 62-66 kDa |
GenBank Accession Number | BC032751 |
Gene Symbol | GFI1 |
Gene ID (NCBI) | 2672 |
RRID | AB_2109976 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q99684 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GFI is a transcriptional repressor that is transiently induced during T-cell differentiation. It has critical roles in enhancing T helper-2 (Th2) cell expansion and in repressing induction of Th17 and CD103-positive inducible regulatory T (Treg) cells [PMID: 19188499]. GFI1 transcriptional regulator oncoprotein plays a crucial role in hematopoietic, inner ear, and pulmonary neuroendocrine cell development and governs cell processes as diverse as self-renewal of hematopoietic stem cells, proliferation, apoptosis, differentiation, cell fate specification, and oncogenesis (PMID: 18805794). The calculated molecular weight of GFI1 protein is about 45 kDa, but the modification of ubiquitinated GIF1 protein is about 62-66 kDa (PMID: 12721361).
实验方案
Product Specific Protocols | |
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WB protocol for GFI1 antibody 14198-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |