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验证数据展示

  • WB analysis of K-562 using 14198-1-AP

    WB analysis of K-562 using 14198-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14198-1-AP (GFI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

GFI1 Polyclonal antibody

GFI1 Polyclonal Antibody for WB, ELISA
Cat No. 14198-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA and More (1)

Synonyms

GFI 1, GFI1, Zinc finger protein 163, Zinc finger protein Gfi 1, ZNF163

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, HL-60 cells, mouse thymus tissue, PC-3 cells, Raji cells, RAW264.7

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

14198-1-AP targets GFI1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen GFI1 fusion protein Ag5401 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name growth factor independent 1 transcription repressor
Synonyms GFI 1, GFI1, Zinc finger protein 163, Zinc finger protein Gfi 1, ZNF163
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 62-66 kDa
GenBank Accession NumberBC032751
Gene Symbol GFI1
Gene ID (NCBI) 2672
RRIDAB_2109976
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99684
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GFI is a transcriptional repressor that is transiently induced during T-cell differentiation. It has critical roles in enhancing T helper-2 (Th2) cell expansion and in repressing induction of Th17 and CD103-positive inducible regulatory T (Treg) cells [PMID: 19188499]. GFI1 transcriptional regulator oncoprotein plays a crucial role in hematopoietic, inner ear, and pulmonary neuroendocrine cell development and governs cell processes as diverse as self-renewal of hematopoietic stem cells, proliferation, apoptosis, differentiation, cell fate specification, and oncogenesis (PMID: 18805794). The calculated molecular weight of GFI1 protein is about 45 kDa, but the modification of ubiquitinated GIF1 protein is about 62-66 kDa (PMID: 12721361).

实验方案

Product Specific Protocols
WB protocol for GFI1 antibody 14198-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
View Article