GFI1 Polyclonal antibody
GFI1 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, HL-60 cells, mouse thymus tissue, PC-3 cells, Raji cells, RAW264.7 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
14198-1-AP targets GFI1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Immunogen | GFI1 fusion protein Ag5401 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | growth factor independent 1 transcription repressor |
| Synonyms | GFI 1, GFI1, Zinc finger protein 163, Zinc finger protein Gfi 1, ZNF163 |
| Calculated Molecular Weight | 45 kDa |
| Observed Molecular Weight | 62-66 kDa |
| GenBank Accession Number | BC032751 |
| Gene Symbol | GFI1 |
| Gene ID (NCBI) | 2672 |
| RRID | AB_2109976 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99684 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GFI is a transcriptional repressor that is transiently induced during T-cell differentiation. It has critical roles in enhancing T helper-2 (Th2) cell expansion and in repressing induction of Th17 and CD103-positive inducible regulatory T (Treg) cells [PMID: 19188499]. GFI1 transcriptional regulator oncoprotein plays a crucial role in hematopoietic, inner ear, and pulmonary neuroendocrine cell development and governs cell processes as diverse as self-renewal of hematopoietic stem cells, proliferation, apoptosis, differentiation, cell fate specification, and oncogenesis (PMID: 18805794). The calculated molecular weight of GFI1 protein is about 45 kDa, but the modification of ubiquitinated GIF1 protein is about 62-66 kDa (PMID: 12721361).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GFI1 antibody 14198-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
