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GFI1 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB

Cat No : 61553,61554 61553

Synonyms

GFI1, Growth Factor Independent 1 Transcription Repressor, Growth Factor Independent 1, ZNF163, GFI-1, Growth Factor Independent Protein 1, GFI1A, Zinc Finger Protein 163, Growth Factor Independence-1, SCN2, Zinc Finger Protein Gfi-1, transcription, transcription repressor, hdac1, pab, polyclonal, antibody, western blotting, wb, sample

验证数据展示

  • GFI1 antibody (pAb) tested by Western blot. Nuclear extract of THP-1 cells (20 ug) probed with GFI1 antibody (pAb) at a dilution of 1:500.

    GFI1 antibody (pAb) tested by Western blot. Nuclear extract of THP-1 cells (20 ug) probed with GFI1 antibody (pAb) at a dilution of 1:500.

产品信息

Tested Applications WB

Applications Validated by Active Motif: WB: 1:500 - 1:1,000 dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.
Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This antibody was raised against a peptide within the N-terminal region of human GFI1.
Full Name GFI1 antibody (pAb)
Synonyms GFI1, Growth Factor Independent 1 Transcription Repressor, Growth Factor Independent 1, ZNF163, GFI-1, Growth Factor Independent Protein 1, GFI1A, Zinc Finger Protein 163, Growth Factor Independence-1, SCN2, Zinc Finger Protein Gfi-1, transcription, transcription repressor, hdac1, pab, polyclonal, antibody, western blotting, wb, sample
Molecular weight 55 kDa
GenBank accession numberNP_005254
RRIDAB_2753153
Purification Method Affinity Purified
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

GFI1 (growth factor independent 1 transcription repressor) is a transcription repressor essential for hematopoiesis. Functions in a cell-context and development-specific manner. Binds to 5'-TAAATCAC[AT]GCA-3' in the promoter region of a large number of genes. Component of several complexes, including the EHMT2-GFI1-HDAC1, AJUBA-GFI1-HDAC1 and RCOR-GFI-KDM1A-HDAC complexes, that suppress, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Regulates neutrophil differentiation, promotes proliferation of lymphoid cells, and is required for granulocyte development. Mediates, together with U2AF1L4, the alternative splicing of CD45 and controls T-cell receptor signaling. Regulates the endotoxin-mediated Toll-like receptor (TLR) inflammatory response by antagonizing RELA. Cooperates with CBFA2T2 to regulate ITGB1-dependent neurite growth. Controls cell-cycle progression by repressing CDKNIA/p21 transcription in response to TGFB1 via recruitment of GFI1 by ZBTB17 to the CDKNIA/p21 and CDKNIB promoters. Required for the maintenance of inner ear hair cells.