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GFRA3 Polyclonal antibody

GFRA3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25379-1-AP

Print datasheet

Synonyms

GDNF family receptor alpha 3, GDNF receptor alpha 3, GDNFR alpha 3, GFR alpha 3, GFRA3

验证数据展示

  • IHC staining of human heart using 25379-1-AP

    IHC staining of human heart using 25379-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25379-1-AP (GFRA3 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human heart using 25379-1-AP

    IHC staining of human heart using 25379-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25379-1-AP (GFRA3 antibody) at dilution of 1:200 (under 40x lens).

  • WB analysis of mouse heart using 25379-1-AP

    WB analysis of mouse heart using 25379-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 25379-1-AP (GFRA3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse heart tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25379-1-AP is GFRA3 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen GFRA3 fusion protein Ag13253 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GDNF family receptor alpha 3
Synonyms GDNF family receptor alpha 3, GDNF receptor alpha 3, GDNFR alpha 3, GFR alpha 3, GFRA3
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC037951
Gene Symbol GFRA3
Gene ID (NCBI) 2676
RRIDAB_2880050
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60609
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GFRA3 is a receptor for the glial cell line-derived neurotrophic factor, ARTN (artemin). It mediates the artemin-induced autophosphorylation and activation of the RET receptor tyrosine kinase. GFRA3 has two isoforms with MW 45 kDa and 41 kDa. It can be cleaved to be a mature form with MW 41 kDa or 37 kDa.

实验方案

Product Specific Protocols
WB protocol for GFRA3 antibody 25379-1-APDownload protocol
IHC protocol for GFRA3 antibody 25379-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols