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  • Featured Product
  • KD/KO Validated

GGA1 Polyclonal antibody

GGA1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 25674-1-AP

Print datasheet

Synonyms

GGA1

验证数据展示

  • IF Staining of HepG2 using 25674-1-AP

    IF Staining of HepG2 using 25674-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 25674-1-AP (GGA1 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis of HEK-293 using 25674-1-AP

    WB analysis of HEK-293 using 25674-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25674-1-AP (GGA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis using 25674-1-AP

    WB analysis using 25674-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25674-1-AP (GGA1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, mouse brain tissue, rat brain tissue, HeLa cells, SH-SY5Y cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25674-1-AP targets GGA1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, rat, mouse
Cited Reactivityhuman, mouse
Immunogen GGA1 fusion protein Ag22614 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name golgi associated, gamma adaptin ear containing, ARF binding protein 1
Synonyms GGA1
Observed Molecular Weight 70-72 kDa
GenBank Accession NumberBC044629
Gene Symbol GGA1
Gene ID (NCBI) 26088
RRIDAB_2880188
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UJY5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GGA1 antibody 25674-1-APDownload protocol
IF protocol for GGA1 antibody 25674-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Clin Invest

Chromosomal 3q amplicon encodes essential regulators of secretory vesicles that drive secretory addiction in cancer

Authors - Xiaochao Tan
View Article
humanWB,IF

J Biol Chem

GGA1 interacts with the endosomal Na+/H+ exchanger NHE6 governing localization to the endosome compartment

Authors - Li Ma
View Article
humanWB

Cell Rep

Golgi retention and oncogenic KIT signaling via PLCγ2-PKD2-PI4KIIIβ activation in gastrointestinal stromal tumor cells

Authors - Yuuki Obata
  • KD Validated
View Article
mouseWB

Environ Toxicol

Total flavonoids of Rhizoma drynariae improves tendon-bone healing for anterior cruciate ligament reconstruction in mice and promotes the osteogenic differentiation of bone mesenchymal stem cells by the ERR1/2-Gga1-TGF-β/MAPK pathway

Authors - Lei Han
View Article
humanWB

Sci Adv

ER-export and ARFRP1/AP-1-dependent delivery of SARS-CoV-2 Envelope to lysosomes controls late stages of viral replication

Authors - Guy J Pearson
View Article