验证数据展示
经过测试的应用
| Positive WB detected in | MCF7 cells, HEK-293 cells, HepG2 cells, L02 cells, MCF-7 cells, mouse liver tissue, PC-3 cells |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human liver tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16209-1-AP targets GGCX in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | GGCX fusion protein Ag9185 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | gamma-glutamyl carboxylase |
| 别名 | gamma glutamyl carboxylase, GC, GGCX, VKCFD1 |
| 计算分子量 | 758 aa, 88 kDa |
| 观测分子量 | 88 kDa |
| GenBank蛋白编号 | BC013979 |
| 基因名称 | GGCX |
| Gene ID (NCBI) | 2677 |
| RRID | AB_2110874 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P38435 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GGCX(Gamma-glutamyl carboxylase) is also named as GC and belongs to the vitamin K-dependent gamma-carboxylase family. This 94 kDa (including all modifications, such as the five N-linked glycosylations), is a 5-pass transmembrane protein and a key regulator of blood coagulation(PMID:20518534). It mediates the vitamin K-dependent carboxylation of glutamate residues to calcium-binding gamma-carboxyglutamate (Gla) residues with the concomitant conversion of the reduced hydroquinone form of vitamin K to vitamin K epoxide. Defects in GGCX are a cause of combined deficiency of vitamin K-dependent clotting factors type 1 (VKCFD1) and pseudoxanthoma elasticum-like disorder with multiple coagulation factor deficiency (PXEL-MCFD)(PMID:9845520;17110937).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GGCX antibody 16209-1-AP | Download protocol |
| IHC protocol for GGCX antibody 16209-1-AP | Download protocol |
| IP protocol for GGCX antibody 16209-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Blood Characterization of vitamin K-dependent carboxylase mutations that cause bleeding and nonbleeding disorders. | ||
Methods Enzymol Functional Study of the Vitamin K Cycle Enzymes in Live Cells.
| ||
JCI Insight VKOR paralog VKORC1L1 supports vitamin K-dependent protein carboxylation in vivo. | ||
Oncotarget Divergent effects of vitamins K1 and K2 on triple negative breast cancer cells. | ||
Cell Rep Vitamin K-dependent carboxylation regulates Ca2+ flux and adaptation to metabolic stress in β cells |
