GGH Polyclonal antibody
GGH Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, MCF-7 cells, HepG2 cells, HT-1080 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy GGH Ready-To-Use IHC Kit. GGH primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13264-1-AP targets GGH in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | GGH fusion protein Ag3939 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase) |
| Synonyms | Conjugase, Gamma Glu X carboxypeptidase, Gamma glutamyl hydrolase, GGH, GH |
| Calculated Molecular Weight | 318 aa, 36 kDa |
| Observed Molecular Weight | 30-37 kDa, 55 kDa |
| GenBank Accession Number | BC025025 |
| Gene Symbol | GGH |
| Gene ID (NCBI) | 8836 |
| RRID | AB_2877931 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92820 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GGH (Gamma glutamyl hydrolase), also named as GH or Conjugase, is a key lysosomal enzyme involved in the metabolism of folic acid and in the action of antifolate drugs (PMID: 16945597). GGH catalyzes the removal of γ-linked polyglutamates from the intracellular folylpolyglutamates to yield folylmonoglutamate coenzymes (PMID: 9614206). The full-length protein has a calculated molecular mass of 36 kDa, contains four potential asparagine glycosylation sites, and was predicted to have a 24-amino-acid signal peptide (PMID: 8816764). GGH can form homodimer which contains two potential active sites (PMID: 16945597). Some bands can be detected by SDS-PAGE: 35-37kDa (full-length), 55kDa (glycosylated form) and 30-33kDa (signal peptide removed) (PMID: 8621474).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GGH antibody 13264-1-AP | Download protocol |
| IHC protocol for GGH antibody 13264-1-AP | Download protocol |
| IP protocol for GGH antibody 13264-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Invest Chromosomal 3q amplicon encodes essential regulators of secretory vesicles that drive secretory addiction in cancer | ||
Front Pharmacol Up-regulation of ABCG1 is associated with methotrexate resistance in acute lymphoblastic leukemia cells | ||
Reprod Sci The Identification of Gamma-Glutamyl Hydrolase in Uterine Corpus Endometrial Carcinoma: a Predictive Model and Machine Learning |
