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验证数据展示

  • WB analysis using 30345-1-AP

    WB analysis using 30345-1-AP

    Human placenta tissue were subjected to SDS PAGE followed by western blot with 30345-1-AP (GH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 30345-1-AP

    IHC staining of human placenta using 30345-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30345-1-AP (Growth Hormone antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 30345-1-AP

    IHC staining of human placenta using 30345-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30345-1-AP (Growth Hormone antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Growth Hormone Polyclonal antibody

Growth Hormone Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30345-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

GH, GH1, growth hormone 1, GH-N, GH 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30345-1-AP targets Growth Hormone in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 fusion protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GH1
别名 GH, GH1, growth hormone 1, GH-N, GH 1
计算分子量 217 aa, 25 kDa
观测分子量22 kDa
GenBank蛋白编号BC075012
基因名称 GH1
Gene ID (NCBI) 2688
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP01241
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GH1, also named as GH and GH-N, belongs to the somatotropin/prolactin family. GH1 plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.

实验方案

Product Specific Protocols
WB protocol for Growth Hormone antibody 30345-1-APDownload protocol
IHC protocol for Growth Hormone antibody 30345-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols