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GH1 Polyclonal antibody

GH1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 55243-1-AP

Print datasheet

Synonyms

GH, GH N, GH1, GHN, hGH N, Somatotropin

验证数据展示

  • WB analysis of human placenta using 55243-1-AP

    WB analysis of human placenta using 55243-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 55243-1-AP (GH1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human pituitary using 55243-1-AP

    IHC staining of human pituitary using 55243-1-AP

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 55243-1-AP (GH1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pituitary using 55243-1-AP

    IHC staining of human pituitary using 55243-1-AP

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 55243-1-AP (GH1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman pituitary tissue, human pituitary adenoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55243-1-AP targets GH1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivitymouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GH1
Synonyms GH, GH N, GH1, GHN, hGH N, Somatotropin
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberNM_000515
Gene Symbol GH1
Gene ID (NCBI) 2688
RRIDAB_11182710
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01241
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

GH1, also named as GH and GH-N, belongs to the somatotropin/prolactin family. GH1 plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues. This antibody has no cross reaction to CSH1.

实验方案

Product Specific Protocols
WB protocol for GH1 antibody 55243-1-APDownload protocol
IHC protocol for GH1 antibody 55243-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratIF

Metabolism

PMID: 31666193

Authors - Jian Mao
View Article
mouseWB,IF

Biochem Biophys Res Commun

Elongator regulates the melanocortin satiety pathway.

Authors - Joseph Walters
View Article
ratWB

Toxicol Lett

The disrupting effect of chlormequat chloride on growth hormone is associated with pregnancy

Authors - Zongzhen Wu
View Article