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GHDC Polyclonal antibody

GHDC Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat No : 26123-1-AP

Print datasheet

Synonyms

D11LGP1, D11LGP1E, GH3 domain containing, GH3 domain containing protein, GHDC, LGP1

验证数据展示

  • WB analysis of HEK-293 using 26123-1-AP

    WB analysis of HEK-293 using 26123-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 26123-1-AP (GHDC Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 26123-1-AP

    IHC staining of human brain using 26123-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26123-1-AP (GHDC Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with GHDC antibody included
  • IHC staining of human brain using 26123-1-AP

    IHC staining of human brain using 26123-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26123-1-AP (GHDC Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with GHDC antibody included
  • IF Staining of HEK-293 using 26123-1-AP

    IF Staining of HEK-293 using 26123-1-AP

    Immunofluorescent analysis of HEK-293 cells using 26123-1-AP (GHDC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman brain tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy GHDC Ready-To-Use IHC Kit. GHDC primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 26123-1-AP is GHDC Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen GHDC fusion protein Ag23480 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GH3 domain containing
Synonyms D11LGP1, D11LGP1E, GH3 domain containing, GH3 domain containing protein, GHDC, LGP1
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC022784
Gene Symbol GHDC
Gene ID (NCBI) 84514
RRIDAB_2880391
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N2G8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GHDC antibody 26123-1-APDownload protocol
IHC protocol for GHDC antibody 26123-1-APDownload protocol
IF protocol for GHDC antibody 26123-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols