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GHITM Recombinant antibody, PBS Only (Detector)

GHITM Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240541D3

Cat no : 83548-3-PBS

Print datasheet

Synonyms

My021, MICS1, Growth hormone-inducible transmembrane protein, DERP2, Dermal papilla-derived protein 2

验证数据展示

  • WB analysis using 83548-3-RR (same clone as 83548-3-PBS)

    WB analysis using 83548-3-RR (same clone as 83548-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83548-3-RR (GHITM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83548-3-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83548-3-RR (same clone as 83548-3-PBS)

    FC experiment of HeLa using 83548-3-RR (same clone as 83548-3-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug GHITM Recombinant antibody (83548-3-RR, Clone:240541D3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83548-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00524-1

    Cytometric bead array standard curve of MP00524-1

    Cytometric bead array standard curve of MP00524-1, GHITM Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83548-2-PBS. Detection antibody: 83548-3-PBS. Standard: Ag35170. Range: 0.78-100 ng/mL.

  • Cytometric bead array standard curve of MP00524-2

    Cytometric bead array standard curve of MP00524-2

    Cytometric bead array standard curve of MP00524-2, GHITM Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83548-1-PBS. Detection antibody: 83548-3-PBS. Standard: Ag35170. Range: 0.78-100 ng/mL.

  • Affinity and Kinetic Characterization of 83548-3-RR

    Affinity and Kinetic Characterization of 83548-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83548-3-RR against Human GHITM were performed. The affinity constant is 2.41 nM.

产品信息

83548-3-PBS targets GHITM as part of a matched antibody pair:

MP00524-1: 83548-2-PBS capture and 83548-3-PBS detection (validated in Cytometric bead array)

MP00524-2: 83548-1-PBS capture and 83548-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen GHITM fusion protein Ag35170 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name growth hormone inducible transmembrane protein
Synonyms My021, MICS1, Growth hormone-inducible transmembrane protein, DERP2, Dermal papilla-derived protein 2
Calculated Molecular Weight 345 aa, 37 kDa
Observed Molecular Weight37 kDa
GenBank Accession NumberBC010354
Gene Symbol GHITM
Gene ID (NCBI) 27069
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H3K2
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GHITM, also known as MICS1, TMBIM5 or DERP2, is a mitochondrial protein that localizes in the inner membrane. GHITM is involved in mitochondrial morphology in specific cristae structures and the apoptotic release of cytochrome c from the mitochondria (PMID: 18417609). The gene of GHITM maps to chromosome 10q23.1, and encodes a 345-amino-acid protein with a calculated molecular mass of 37 kDa.