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GHR Polyclonal antibody

GHR Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20713-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, pig and More (1)

应用

WB, IHC, IF/ICC, ELISA

Somatotropin receptor, Serum binding protein, Growth hormone-binding protein, GHBP, GH receptor

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inpig liver tissue, pig lung tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20713-1-AP targets GHR in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, pig
文献引用反应性human, pig, rabbit
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GH binding protein
别名 Somatotropin receptor, Serum binding protein, Growth hormone-binding protein, GHBP, GH receptor
计算分子量 72 kDa
观测分子量 120 kDa
GenBank蛋白编号NM_000163
基因名称 GHR
Gene ID (NCBI) 2690
RRIDAB_2878726
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP10912
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GH is a peptide hormone with many pleiotropic actions, including the regulation of metabolism, postnatal growth, cognition, immune, cardiac and renal systems and gut function (PMID: 26059750). GH exerts these actions primarily through alterations in gene expression, initiated by activation of its membrane receptor GHR and the resultant activation of the associated JAK2 and Src family kinases. GH receptor (GHR) is a member of the cytokine receptor family. Mutations in GHR gene have been associated with Laron syndrome, also known as the GH insensitivity syndrome (GHIS), a disorder characterized by short stature.

实验方案

Product Specific Protocols
WB protocol for GHR antibody 20713-1-APDownload protocol
IHC protocol for GHR antibody 20713-1-APDownload protocol
IF protocol for GHR antibody 20713-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Pharmacol Res

Discovery of a novel megakaryopoiesis enhancer, ingenol, promoting thrombopoiesis through PI3K-Akt signaling independent of thrombopoietin.

Authors - Long Wang
humanWB

J Cell Mol Med

GHR is involved in gastric cell growth and apoptosis via PI3K/AKT signalling.

Authors - Hong-Zhu Yan
  • KD Validated
humanWB

Cell Signal

The inhibition of GHR enhanced cytotoxic effects of etoposide on neuroblastoma.

Authors - Hongmei Zhang
humanWB

FEBS Open Bio

PMID: 31725956

Authors - Mo Cheng
  • KD Validated
humanWB

FEBS Open Bio

PMID: 32069380

Authors - Xiaojue Zhu
  • KD Validated
pigIHC,WB

Front Cell Dev Biol

One-step in vivo gene knock-out in porcine embryos using recombinant adeno-associated viruses

Authors - Mengyu Gao
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