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GIMAP4 Polyclonal antibody

GIMAP4 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11852-1-AP

Print datasheet

Synonyms

Immunity associated protein 4, IMAP4, IAN-1, IAN1, IAN 1

验证数据展示

  • WB analysis of Jurkat using 11852-1-AP

    WB analysis of Jurkat using 11852-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11852-1-AP (GIMAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 11852-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 11852-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 11852-1-AP (GIMAP4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of Jurkat using 11852-1-AP

    IP experiment of Jurkat using 11852-1-AP

    IP result of anti-GIMAP4 (IP:11852-1-AP, 3ug; Detection:11852-1-AP 1:800) with Jurkat cells lysate 1080ug.

经过测试的应用

Positive WB detected inJurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11852-1-AP targets GIMAP4 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Tested Reactivity human
Immunogen GIMAP4 fusion protein Ag2422 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GTPase, IMAP family member 4
Synonyms Immunity associated protein 4, IMAP4, IAN-1, IAN1, IAN 1
Calculated Molecular Weight 329 aa, 38 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC020657
Gene Symbol GIMAP4
Gene ID (NCBI) 55303
RRIDAB_2279105
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NUV9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GIMAP4, also named as hIAN1 or HIMAP4, is a member of GIMAP family. Among GIMAP family, GIMAP4 is the only member reported to have true GTPase activity, and its transcription is found to be differentially regulated during early human CD4+ T helper (Th) lymphocyte differentiation. GIMAP4 has been previously connected mainly with T- and B-cell development and survival and T-cell apoptosis. The MW of this protein is 38 kDa, and Catalog#11852-1-AP specially recognises the 38 kDa protein.

实验方案

Product Specific Protocols
WB protocol for GIMAP4 antibody 11852-1-APDownload protocol
IHC protocol for GIMAP4 antibody 11852-1-APDownload protocol
IP protocol for GIMAP4 antibody 11852-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols