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验证数据展示

  • WB analysis of Jurkat using 11852-1-AP

    WB analysis of Jurkat using 11852-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11852-1-AP (GIMAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 11852-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 11852-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 11852-1-AP (GIMAP4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of Jurkat using 11852-1-AP

    IP experiment of Jurkat using 11852-1-AP

    IP result of anti-GIMAP4 (IP:11852-1-AP, 3ug; Detection:11852-1-AP 1:800) with Jurkat cells lysate 1080ug.

GIMAP4 Polyclonal antibody

GIMAP4 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 11852-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, ELISA

别名

Immunity associated protein 4, IMAP4, IAN-1, IAN1, IAN 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11852-1-AP targets GIMAP4 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human
免疫原 GIMAP4 fusion protein Ag2422 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GTPase, IMAP family member 4
别名 Immunity associated protein 4, IMAP4, IAN-1, IAN1, IAN 1
计算分子量 329 aa, 38 kDa
观测分子量 38 kDa
GenBank蛋白编号BC020657
基因名称 GIMAP4
Gene ID (NCBI) 55303
RRIDAB_2279105
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NUV9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GIMAP4, also named as hIAN1 or HIMAP4, is a member of GIMAP family. Among GIMAP family, GIMAP4 is the only member reported to have true GTPase activity, and its transcription is found to be differentially regulated during early human CD4+ T helper (Th) lymphocyte differentiation. GIMAP4 has been previously connected mainly with T- and B-cell development and survival and T-cell apoptosis. The MW of this protein is 38 kDa, and Catalog#11852-1-AP specially recognises the 38 kDa protein.

实验方案

Product Specific Protocols
WB protocol for GIMAP4 antibody 11852-1-APDownload protocol
IHC protocol for GIMAP4 antibody 11852-1-APDownload protocol
IP protocol for GIMAP4 antibody 11852-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols