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GINS1 Polyclonal antibody

GINS1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16061-1-AP

Print datasheet

Synonyms

PSF1, KIAA0186, GINS complex subunit 1

验证数据展示

  • WB analysis using 16061-1-AP

    WB analysis using 16061-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16061-1-AP (GINS1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 16061-1-AP

    IHC staining of human ovary cancer using 16061-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16061-1-AP (GINS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inMCF-7 cells, SH-SY5Y cells, U-251 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16061-1-AP targets GINS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen GINS1 fusion protein Ag8895 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GINS complex subunit 1 (Psf1 homolog)
Synonyms PSF1, KIAA0186, GINS complex subunit 1
Calculated Molecular Weight 196 aa, 23 kDa
Observed Molecular Weight20 kDa
GenBank Accession NumberBC012542
Gene Symbol GINS1
Gene ID (NCBI) 9837
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14691
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GINS1, also known as Partner of SLD Five 1 (PSF1) is a member of the GINS (Go-Ichi-Nii-San) complex, which plays a vital role in the initiation and elongation of DNA replication (PMID: 34414190). GINS1 was initially found to be predominantly expressed in highly proliferating cells instead of mature cells. Later on, it was found that GINS was involved in the tumorigenesis of various cancers, including breast cancer, colorectal cancer, and hepatocellular carcinoma (PMID: 36065190).

实验方案

Product Specific Protocols
WB protocol for GINS1 antibody 16061-1-APDownload protocol
IHC protocol for GINS1 antibody 16061-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols