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验证数据展示

  • WB analysis using 28322-1-AP

    WB analysis using 28322-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 28322-1-AP (GIPR antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis using 28322-1-AP

    WB analysis using 28322-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 28322-1-AP (GIPR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 28322-1-AP

    IHC staining of mouse brain using 28322-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28322-1-AP (GIPR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 28322-1-AP

    IHC staining of mouse brain using 28322-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28322-1-AP (GIPR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GIPR Polyclonal antibody

GIPR Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28322-1-AP

Print datasheet

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Glucose-dependent insulinotropic polypeptide receptor, GIP-R, GIP R

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHT-1080 cells, K-562 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

28322-1-AP targets GIPR in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen GIPR fusion protein Ag28143 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name gastric inhibitory polypeptide receptor
Synonyms Glucose-dependent insulinotropic polypeptide receptor, GIP-R, GIP R
Calculated Molecular Weight 466 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC093723
Gene Symbol GIPR
Gene ID (NCBI) 2696
RRIDAB_2881113
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP48546
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GIPR, or Gastric Inhibitory Polypeptide Receptor, plays a crucial role in glucose metabolism and insulin secretion. It is a G protein-coupled receptor (GPCR). It belongs to the B1 class of this receptor family, which is involved in various physiological processes including fat generation, pancreatic beta-cell proliferation, and insulin release.

实验方案

Product Specific Protocols
WB protocol for GIPR antibody 28322-1-APDownload protocol
IHC protocol for GIPR antibody 28322-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

PLoS One

Gut-derived peptide hormone receptor expression in the developing mouse hypothalamus

Authors - Lídia Cantacorps
View Article