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GIT1 Polyclonal antibody

GIT1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26247-1-AP

Print datasheet

Synonyms

Cool-associated and tyrosine-phosphorylated protein 1, CAT-1, CAT1, CAT 1, ARF GTPase-activating protein GIT1

验证数据展示

  • WB analysis of ROS1728 using 26247-1-AP

    WB analysis of ROS1728 using 26247-1-AP

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 26247-1-AP (GIT1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inROS1728 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26247-1-AP targets GIT1 in WB, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, rat
Immunogen GIT1 fusion protein Ag24648 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name G protein-coupled receptor kinase interacting ArfGAP 1
Synonyms Cool-associated and tyrosine-phosphorylated protein 1, CAT-1, CAT1, CAT 1, ARF GTPase-activating protein GIT1
Calculated Molecular Weight 694 aa, 77 kDa
Observed Molecular Weight80 kDa
GenBank Accession NumberBC067358
Gene Symbol GIT1
Gene ID (NCBI) 28964
RRIDAB_2880445
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2X7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GIT1 (ARF GTPase-activating protein GIT1) is a member of the GIT subfamily of ADP ribosylation factor (ARF)-GTPase activating protein (GAP) family of proteins, which are characterized by an ARFGAP domain that stimulates the GTPase activity of proteins. GIT1 was highly expressed in the nervous system, and its expression was maintained throughout all brain neuritogenesis stages (PMID: 27127481).

实验方案

Product Specific Protocols
WB protocol for GIT1 antibody 26247-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols