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验证数据展示

  • WB analysis of ROS1728 using 26247-1-AP

    WB analysis of ROS1728 using 26247-1-AP

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 26247-1-AP (GIT1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

GIT1 Polyclonal antibody

GIT1 Polyclonal Antibody for WB, ELISA
Cat No. 26247-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, ELISA

别名

Cool-associated and tyrosine-phosphorylated protein 1, CAT-1, CAT1, CAT 1, ARF GTPase-activating protein GIT1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inROS1728 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26247-1-AP targets GIT1 in WB, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, rat
免疫原 GIT1 fusion protein Ag24648 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor kinase interacting ArfGAP 1
别名 Cool-associated and tyrosine-phosphorylated protein 1, CAT-1, CAT1, CAT 1, ARF GTPase-activating protein GIT1
计算分子量 694 aa, 77 kDa
观测分子量80 kDa
GenBank蛋白编号BC067358
基因名称 GIT1
Gene ID (NCBI) 28964
RRIDAB_2880445
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y2X7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GIT1 (ARF GTPase-activating protein GIT1) is a member of the GIT subfamily of ADP ribosylation factor (ARF)-GTPase activating protein (GAP) family of proteins, which are characterized by an ARFGAP domain that stimulates the GTPase activity of proteins. GIT1 was highly expressed in the nervous system, and its expression was maintained throughout all brain neuritogenesis stages (PMID: 27127481).

实验方案

Product Specific Protocols
WB protocol for GIT1 antibody 26247-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols