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验证数据展示

  • IHC staining of mouse lung using 26602-1-AP

    IHC staining of mouse lung using 26602-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26602-1-AP (GJA5/Connexin 40 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse lung using 26602-1-AP

    IHC staining of mouse lung using 26602-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26602-1-AP (Connexin 40 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse lung using 26602-1-AP

    IF Staining of mouse lung using 26602-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using GJA5/Connexin 40 antibody (26602-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GJA5/Connexin 40 Polyclonal antibody

GJA5/Connexin 40 Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 26602-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF-P, ELISA

别名

Connexin 40, GJA5, Connexin-40, CX40, Gap junction alpha 5 protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse lung tissue

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26602-1-AP targets GJA5/Connexin 40 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 GJA5/Connexin 40 fusion protein Ag24266 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 gap junction protein, alpha 5, 40kDa
别名 Connexin 40, GJA5, Connexin-40, CX40, Gap junction alpha 5 protein
计算分子量 40 kDa
GenBank蛋白编号BC013313
基因名称 GJA5
Gene ID (NCBI) 2702
RRIDAB_3669552
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP36382
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Connexin 40 (Cx40), also known as Gap junction alpha 5 protein (GJA5), belongs to the connexin family. Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. Four connexins (Cxs) are expressed in the cardiovascular system (Cx37, Cx40, Cx43, and Cx45) and are involved in the development of the cardiovascular system(PMID: 37331352; PMID: 17922338).

实验方案

Product Specific Protocols
IHC protocol for GJA5/Connexin 40 antibody 26602-1-APDownload protocol
IF protocol for GJA5/Connexin 40 antibody 26602-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols