Connexin-32 Polyclonal antibody

Connexin-32 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 10450-1-AP

Print datasheet

Synonyms

GJB1, Gap junction beta-1 protein, Gap junction beta 1 protein, CX32, CMTX1



经过测试的应用

Positive WB detected inmouse brain tissue, A375 cells, human liver tissue, mouse skin tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10450-1-AP targets Connexin-32 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen Connexin-32 fusion protein Ag0659 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name gap junction protein, beta 1, 32kDa
Synonyms GJB1, Gap junction beta-1 protein, Gap junction beta 1 protein, CX32, CMTX1
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC002805
Gene Symbol Connexin-32
Gene ID (NCBI) 2705
RRIDAB_2110629
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08034
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Connexin-32 (Cx32), also known as Gap junction beta 1 protein (GJB1), belongs to the connexin family. Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. Connexin-32 is the major gap junction protein of liver, and the expression is also found in the brain and other organs. It is involved in tumorigenesis and liver regeneration. Connexin-32 plays an important role in peripheral nerve. Defects in Connexin-32 are the cause of Charcot-Marie-Tooth disease X-linked type 1 (CMTX1), an inherited peripheral neuropathy. Cx32 protein can be detected as a 27-32 kDa monomer and a 48-54 kDa dimer (PMID: 8266101; 8613761; 8608591; 19265674; 17972320; 19845480)

实验方案

Product Specific Protocols
WB protocol for Connexin-32 antibody 10450-1-APDownload protocol
IHC protocol for Connexin-32 antibody 10450-1-APDownload protocol
IP protocol for Connexin-32 antibody 10450-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Chemosphere

Organophosphate flame retardant TDCPP: A risk factor for renal cancer?

Authors - Xuan Zhou
humanWB

World J Gastroenterol

PBX1 attributes as a determinant of connexin 32 downregulation in Helicobacter pylori-related gastric carcinogenesis.

Authors - Xiao-Ming Liu
mouseWB

Brain Res Bull

Transient receptor potential vanilloid 4 is involved in the upregulation of connexin expression following pilocarpine-induced status epilepticus in mice.

Authors - Chen Men
humanIHC

J Biomed Biotechnol

Ischemia alters the expression of connexins in the aged human brain.

Authors - Nakase Taizen T
humanIHC, IF

Histopathology

Down-regulation of connexin43 and connexin32 in keratocystic odontogenic tumours: potential association with clinical features.

Authors - Wen-Qun Zhong
humanWB

Oncol Lett

Effects of Helicobacter pylori on the expression levels of GATA-3 and connexin 32 and the GJIC function in gastric epithelial cells and their association by promoter analysis.

Authors - Lihua Huang