Connexin-32 Polyclonal antibody
Connexin-32 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, A375 cells, human liver tissue, mouse skin tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human liver cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10450-1-AP targets Connexin-32 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Immunogen | Connexin-32 fusion protein Ag0659 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | gap junction protein, beta 1, 32kDa |
Synonyms | GJB1, Gap junction beta-1 protein, Gap junction beta 1 protein, CX32, CMTX1 |
Calculated Molecular Weight | 32 kDa |
Observed Molecular Weight | 32 kDa |
GenBank Accession Number | BC002805 |
Gene Symbol | Connexin-32 |
Gene ID (NCBI) | 2705 |
RRID | AB_2110629 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P08034 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Connexin-32 antibody 10450-1-AP | Download protocol |
IHC protocol for Connexin-32 antibody 10450-1-AP | Download protocol |
IP protocol for Connexin-32 antibody 10450-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
World J Gastroenterol PBX1 attributes as a determinant of connexin 32 downregulation in Helicobacter pylori-related gastric carcinogenesis. | ||
Brain Res Bull Transient receptor potential vanilloid 4 is involved in the upregulation of connexin expression following pilocarpine-induced status epilepticus in mice. | ||
Histopathology Down-regulation of connexin43 and connexin32 in keratocystic odontogenic tumours: potential association with clinical features. | ||
Oncol Lett Effects of Helicobacter pylori on the expression levels of GATA-3 and connexin 32 and the GJIC function in gastric epithelial cells and their association by promoter analysis. |