验证数据展示
经过测试的应用
Positive IHC detected in | human oesophagus tissue, human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
24215-1-AP targets GJB6 in IHC, ELISA applications and shows reactivity with human samples.
经测试应用 | IHC, ELISA Application Description |
经测试反应性 | human |
免疫原 | GJB6 fusion protein Ag21529 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | gap junction protein, beta 6, 30kDa |
别名 | Connexin 30, CX30, DFNA3, DFNA3B, DFNB1B, ED2, EDH, Gap junction beta 6 protein, GJB6, HED |
计算分子量 | 261 aa, 30 kDa |
GenBank蛋白编号 | BC038934 |
基因名称 | GJB6 |
Gene ID (NCBI) | 10804 |
RRID | AB_2879461 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen Affinity purified |
UNIPROT ID | O95452 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Gap junctions form conduits between adjacent cells that are composed of connexin protein subunits and provide direct intercellular communication pathways allowing rapid exchange of ions and metabolites (PMID: 12126230; 15094343). Connexins are four-pass transmembrane proteins with amino- and carboxy-terminal regions facing the cytoplasm. A connexon is composed of a hexamer of connexins. GJB6 (gap junction beta-6 protein, also known as connexin-30) is a member of the connexin family of proteins. GJB6 is a component of the gap junction networks of the cochlea.
实验方案
Product Specific Protocols | |
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IHC protocol for GJB6 antibody 24215-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |