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验证数据展示

  • IHC staining of human oesophagus using 24215-1-AP

    IHC staining of human oesophagus using 24215-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 24215-1-AP (GJB6 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human oesophagus using 24215-1-AP

    IHC staining of human oesophagus using 24215-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 24215-1-AP (GJB6 Antibody) at dilution of 1:50 (under 40x lens).

GJB6 Polyclonal antibody

GJB6 Polyclonal Antibody for IHC, ELISA
Cat No. 24215-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Synonyms

Connexin 30, CX30, DFNA3, DFNA3B, DFNB1B, ED2, EDH, Gap junction beta 6 protein, GJB6, HED

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IHC detected inhuman oesophagus tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24215-1-AP targets GJB6 in IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, ELISA Application Description
Tested Reactivity human
Immunogen GJB6 fusion protein Ag21529 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name gap junction protein, beta 6, 30kDa
Synonyms Connexin 30, CX30, DFNA3, DFNA3B, DFNB1B, ED2, EDH, Gap junction beta 6 protein, GJB6, HED
Calculated Molecular Weight 261 aa, 30 kDa
GenBank Accession NumberBC038934
Gene Symbol GJB6
Gene ID (NCBI) 10804
RRIDAB_2879461
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDO95452
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Gap junctions form conduits between adjacent cells that are composed of connexin protein subunits and provide direct intercellular communication pathways allowing rapid exchange of ions and metabolites (PMID: 12126230; 15094343). Connexins are four-pass transmembrane proteins with amino- and carboxy-terminal regions facing the cytoplasm. A connexon is composed of a hexamer of connexins. GJB6 (gap junction beta-6 protein, also known as connexin-30) is a member of the connexin family of proteins. GJB6 is a component of the gap junction networks of the cochlea.

实验方案

Product Specific Protocols
IHC protocol for GJB6 antibody 24215-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols