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验证数据展示

  • WB analysis of human stomach using 14494-1-AP

    WB analysis of human stomach using 14494-1-AP

    human stomach tissue were subjected to SDS PAGE followed by western blot with 14494-1-AP (Gastrokine 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 14494-1-AP

    IHC staining of human stomach using 14494-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 14494-1-AP (Gastrokine 1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human stomach using 14494-1-AP

    IHC staining of human stomach using 14494-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 14494-1-AP (Gastrokine 1 antibody) at dilution of 1:100 (under 40x lens).

  • WB analysis using 14494-1-AP

    WB analysis using 14494-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14494-1-AP (Gastrokine 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Gastrokine 1 Polyclonal antibody

Gastrokine 1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14494-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

18 kDa antrum mucosa protein, AMP 18, AMP18, BRICD1, CA11, FOV, foveolin, gastrokine 1, GKN1, Protein CA11

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman stomach tissue, mouse stomach tissue, rat stomach tissue
Positive IHC detected inhuman stomach tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14494-1-AP targets Gastrokine 1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Gastrokine 1 fusion protein Ag5898 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 gastrokine 1
别名 18 kDa antrum mucosa protein, AMP 18, AMP18, BRICD1, CA11, FOV, foveolin, gastrokine 1, GKN1, Protein CA11
计算分子量 22 kDa
观测分子量 20-22 kDa
GenBank蛋白编号BC059778
基因名称 Gastrokine 1
Gene ID (NCBI) 56287
RRIDAB_10863653
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NS71
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Gastrokine 1 (GKN1), a stomach-specific protein also known as 18 kDa antrum mucosa protein (AMP-18) or foveolin, belongs to the gastrokine family of gastric mucus cell-secreted proteins. The human GKN1 gene has been localized in a region of chromosome 2p13 of about 6 kb and contains 6 exons. GKN1 is expressed only in normal human stomach, but is absent from gastric adenocarcinomas, gastro-esophageal adenocarcinoma cell lines, and other normal and tumor gastro-intestinal tissues. GKN1 may play an important role in normal gastric function and may be a gastric tumour suppressor.

实验方案

Product Specific Protocols
WB protocol for Gastrokine 1 antibody 14494-1-APDownload protocol
IHC protocol for Gastrokine 1 antibody 14494-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols