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验证数据展示

  • WB analysis of human stomach using 60130-1-Ig

    WB analysis of human stomach using 60130-1-Ig

    human stomach tissue were subjected to SDS PAGE followed by western blot with 60130-1-Ig (GKN1 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 60130-1-Ig

    IHC staining of human stomach using 60130-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 60130-1-Ig (GKN1 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human stomach using 60130-1-Ig

    IHC staining of human stomach using 60130-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 60130-1-Ig (GKN1 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of human stomach using 60130-1-Ig

    IF Staining of human stomach using 60130-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human stomach tissue using Gastrokine 1 antibody (60130-1-Ig, Clone: 3C10E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of human stomach using 60130-1-Ig

    IF Staining of human stomach using 60130-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human stomach tissue using Gastrokine 1 antibody (60130-1-Ig, Clone: 3C10E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Gastrokine 1 Monoclonal antibody

Gastrokine 1 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 60130-1-Ig

产品说明书

CloneNo. 3C10E3

宿主/亚型

Mouse / IgG2a

种属反应性

human, rat, pig, mouse

应用

WB, IHC, IF-P, ELISA

别名

18 kDa antrum mucosa protein, AMP 18, AMP18, BRICD1, CA11, FOV, foveolin, gastrokine 1, GKN1, Protein CA11

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman stomach tissue, rat stomach tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman stomach tissue, mouse stomach tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60130-1-Ig targets Gastrokine 1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat, pig, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, rat, pig, mouse
免疫原 Gastrokine 1 fusion protein Ag6091 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 gastrokine 1
别名 18 kDa antrum mucosa protein, AMP 18, AMP18, BRICD1, CA11, FOV, foveolin, gastrokine 1, GKN1, Protein CA11
计算分子量 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC059778
基因名称 Gastrokine 1
Gene ID (NCBI) 56287
RRIDAB_2111459
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NS71
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Gastrokine 1 (GKN1), a stomach-specific protein also known as 18 kDa antrum mucosa protein (AMP-18) or foveolin, belongs to the gastrokine family of gastric mucus cell-secreted proteins. The human GKN1 gene has been localized in a region of chromosome 2p13 of about 6 kb and contains 6 exons. GKN1 is expressed only in normal human stomach, but is absent from gastric adenocarcinomas, gastro-esophageal adenocarcinoma cell lines, and other normal and tumor gastro-intestinal tissues. GKN1may play an important role in normal gastric function and may be a gastric tumour suppressor.

实验方案

Product Specific Protocols
WB protocol for Gastrokine 1 antibody 60130-1-IgDownload protocol
IHC protocol for Gastrokine 1 antibody 60130-1-IgDownload protocol
IF protocol for Gastrokine 1 antibody 60130-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols