验证数据展示
经过测试的应用
| Positive WB detected in | Neuro-2a cells, C6 cells, mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IF/ICC detected in | Neuro-2a cells |
| Positive FC (Intra) detected in | Neuro-2a cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20785-1-AP targets GLAST/EAAT1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, cow |
| 免疫原 | GLAST/EAAT1 fusion protein Ag14177 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 1 (glial high affinity glutamate transporter), member 3 |
| 别名 | EAAT1, GLAST, GLAST-1, SLC1A3, Sodium-dependent glutamate/aspartate transporter 1 |
| 计算分子量 | 542 aa, 60 kDa |
| 观测分子量 | 50-55 kDa, 90-100 kDa |
| GenBank蛋白编号 | BC037310 |
| 基因名称 | GLAST |
| Gene ID (NCBI) | 6507 |
| RRID | AB_2878738 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P43003 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLC1A3, also known as EAAT-1 or GLAST, is a membrane-bound protein localized in glial cells and pre-synaptic glutamatergic nerve endings. It transports the excitatory neurotransmitters L-glutamate and D-aspartate, which is essential for terminating the postsynaptic acction of glutamate. Recently, a correlation between expression/function of glial EAAT-1 and tumor proliferation has been reported. The exceptionally rare expression of EAAT-1 in non-neoplastic choroid plexus (CP) compared to choroid plexus tumors (CPT) may distinguishes neoplastic from normal CP. There are a number of splicing variants of SLC1A3, like GLAST1a and GLAST1b, exist due to the exon skipping. It also undergo glycosylation. Variety of bands can be observed in the western blotting assay: 50-55 kDa represents the unglycosylated GLAST1a or GLAST1b, 65-70 kDa correspond to the glycosylated proteins, larger proteins between 90-130 kDa may be the multimers of SLC1A3. (11086157, 17471058, 12546822)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GLAST/EAAT1 antibody 20785-1-AP | Download protocol |
| IF protocol for GLAST/EAAT1 antibody 20785-1-AP | Download protocol |
| IP protocol for GLAST/EAAT1 antibody 20785-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res H2A.Z.1 crosstalk with H3K56-acetylation controls gliogenesis through the transcription of folate receptor. | ||
Cell Rep SOX2 is essential for astrocyte maturation and its deletion leads to hyperactive behavior in mice | ||
Cell Death Differ RNF20 controls astrocytic differentiation through epigenetic regulation of STAT3 in the developing brain. | ||
J Neuroimmune Pharmacol Brain-Derived Exosomal CircRNAs in Plasma Serve as Diagnostic Biomarkers for Acute Ischemic Stroke | ||
Biochem Pharmacol Regulatory roles of histamine receptor in astrocytic glutamate clearance under conditions of increased glucose variability | ||
Neuropharmacology Ginsenoside Rb1 confers neuroprotection via promotion of glutamate transporters in a mouse model of Parkinson's disease. |
