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验证数据展示

  • WB analysis using 26466-1-AP

    WB analysis using 26466-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26466-1-AP (GLE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 26466-1-AP

    IF Staining of HepG2 using 26466-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 26466-1-AP (GLE1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

GLE1 Polyclonal antibody

GLE1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 26466-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

GLE1, GLE1 like protein, GLE1L, hGLE1, LCCS, LCCS1, Nucleoporin GLE1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A549 cells, MCF-7 cells, PC-3 cells, HepG2 cells, Jurkat cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26466-1-AP targets GLE1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 GLE1 fusion protein Ag24208 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GLE1 RNA export mediator homolog (yeast)
别名 GLE1, GLE1 like protein, GLE1L, hGLE1, LCCS, LCCS1, Nucleoporin GLE1
计算分子量 80 kDa
观测分子量 79-80 kDa
GenBank蛋白编号BC030012
基因名称 GLE1
Gene ID (NCBI) 2733
RRIDAB_2880525
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ53GS7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GLE1 antibody 26466-1-APDownload protocol
IF protocol for GLE1 antibody 26466-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols