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GLE1 Polyclonal antibody

GLE1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26466-1-AP

Print datasheet

Synonyms

GLE1, GLE1 like protein, GLE1L, hGLE1, LCCS, LCCS1, Nucleoporin GLE1

验证数据展示

  • WB analysis using 26466-1-AP

    WB analysis using 26466-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26466-1-AP (GLE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 26466-1-AP

    IF Staining of HepG2 using 26466-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 26466-1-AP (GLE1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, A549 cells, MCF-7 cells, PC-3 cells, HepG2 cells, Jurkat cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26466-1-AP targets GLE1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen GLE1 fusion protein Ag24208 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GLE1 RNA export mediator homolog (yeast)
Synonyms GLE1, GLE1 like protein, GLE1L, hGLE1, LCCS, LCCS1, Nucleoporin GLE1
Calculated Molecular Weight 80 kDa
Observed Molecular Weight 79-80 kDa
GenBank Accession NumberBC030012
Gene Symbol GLE1
Gene ID (NCBI) 2733
RRIDAB_2880525
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ53GS7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GLE1 antibody 26466-1-APDownload protocol
IF protocol for GLE1 antibody 26466-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols