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验证数据展示

  • WB analysis using 66905-1-Ig (same clone as 66905-1-PBS)

    WB analysis using 66905-1-Ig (same clone as 66905-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66905-1-Ig (GLI1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66905-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66905-1-Ig (same clone as 66905-1-PBS)

    IF Staining of HeLa using 66905-1-Ig (same clone as 66905-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GLI1 antibody (66905-1-Ig, Clone: 1D2B2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66905-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

GLI1 Monoclonal antibody, PBS Only

GLI1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66905-1-PBS

产品说明书

CloneNo. 1D2B2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IF/ICC, ELISA

别名

Oncogene GLI, Glioma-associated oncogene, Glioma associated oncogene, GLI family zinc finger 1, GLI

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66905-1-PBS targets GLI1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 GLI1 fusion protein Ag27832 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 GLI family zinc finger 1
别名 Oncogene GLI, Glioma-associated oncogene, Glioma associated oncogene, GLI family zinc finger 1, GLI
计算分子量 1106 aa, 118 kDa
观测分子量 150 kDa
GenBank蛋白编号BC013000
基因名称 GLI1
Gene ID (NCBI) 2735
RRIDAB_2882232
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP08151
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GLI1, also known as Glioma-associated oncogene, is a 1106 amino acid protein, which belongs to the GLI C2H2-type zinc-finger protein family. GLI1 is detected in testis and Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract. GLI1 is tethered in the cytoplasm by binding to SUFU (PubMed:10806483). GLI1 is activated and translocated to the nucleus promoted by interaction with STK36 (PubMed:10806483). Phosphorylation of GLI1 by ULK3 may promote nuclear localization (PubMed:19878745). GLI1 as a transcriptional activator may regulate the transcription of specific genes during normal development (PubMed:19706761). GLI1 plays a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract and Mediates SHH signaling (PubMed:19706761). GLI1 plays a role in cell proliferation and differentiation via its role in SHH signaling.