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验证数据展示

  • IHC staining of human breast cancer using 28245-1-AP

    IHC staining of human breast cancer using 28245-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28245-1-AP (GLI2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GLI2 antibody included
  • WB analysis using 28245-1-AP

    WB analysis using 28245-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28245-1-AP (GLI2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 28245-1-AP

    IHC staining of human breast cancer using 28245-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28245-1-AP (GLI2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GLI2 antibody included

GLI2 Polyclonal antibody

GLI2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28245-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Synonyms

THP2, THP1, THP, Tax helper protein, HPE9

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, U2OS cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy GLI2 Ready-To-Use IHC Kit. GLI2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

28245-1-AP targets GLI2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivitymouse
Immunogen GLI2 fusion protein Ag28422 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GLI family zinc finger 2
Synonyms THP2, THP1, THP, Tax helper protein, HPE9
Calculated Molecular Weight 168 aa
Observed Molecular Weight180-200 kDa
GenBank Accession NumberNM_005270
Gene Symbol GLI2
Gene ID (NCBI) 2736
RRIDAB_3086041
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10070
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GLI2, along with the related GLI1 and GLI3, is a member of the GLI family of transcription factors belonging to the Glis superfamily. Glioma-Associated Oncogene Family Zinc Finger 2 (GLI2) is a zinc-finger transcription factor involved in the Sonic Hedgehog pathway. GLI2 is involved in proliferation, invasion, fibrosis, and is involved in carcinogenesis in various malignancies including gastric cancer, lung cancer, prostate cancer, bladder cancer, and so on.

实验方案

Product Specific Protocols
WB protocol for GLI2 antibody 28245-1-APDownload protocol
IHC protocol for GLI2 antibody 28245-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Proc Natl Acad Sci U S A

Tai/NCOA2 suppresses the Hedgehog pathway by directly targeting the transcription factor Ci/GLI

Authors - Xuan Yu
View Article