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验证数据展示

  • WB analysis of HepG2 using 28272-1-AP

    WB analysis of HepG2 using 28272-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 28272-1-AP (GLI3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 28272-1-AP

    IHC staining of human colon cancer using 28272-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28272-1-AP (GLI3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GLI3 antibody included
  • IHC staining of human prostate cancer using 28272-1-AP

    IHC staining of human prostate cancer using 28272-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28272-1-AP (GLI3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GLI3 antibody included
  • IHC staining of mouse testis using 28272-1-AP

    IHC staining of mouse testis using 28272-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28272-1-AP (GLI3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GLI3 antibody included
  • IHC staining of rat testis using 28272-1-AP

    IHC staining of rat testis using 28272-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28272-1-AP (GLI3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GLI3 antibody included

GLI3 Polyclonal antibody

GLI3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28272-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

GLI3-190, GLI3 full-length protein, GLI3 form of 190 kDa, GLI family zinc finger 3, GCPS

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, SKOV-3 cells
Positive IHC detected inmouse testis tissue, human colon cancer tissue, human prostate cancer tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy GLI3 Ready-To-Use IHC Kit. GLI3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

28272-1-AP targets GLI3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, rat
免疫原 GLI3 fusion protein Ag28472 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GLI family zinc finger 3
别名 GLI3-190, GLI3 full-length protein, GLI3 form of 190 kDa, GLI family zinc finger 3, GCPS
计算分子量 170 aa
观测分子量 170-190 kDa, 80-90 kDa
GenBank蛋白编号NM_000168
基因名称 GLI3
Gene ID (NCBI) 2737
RRIDAB_2881101
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP10071
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GLI3 belongs to the GLI C2H2-type zinc-finger protein family. GLI3 plays a role in limb and brain development. GLI3 is implicated in the transduction of SHH signal. Defects in GLI3 are the cause of Greig cephalo-poly-syndactyly syndrome (GCPS). Defects in GLI3 are a cause of Pallister-Hall syndrome (PHS). Defects in GLI3 are a cause of type A1/B postaxial polydactyly (PAPA1/PAPB). Defects in GLI3 are a cause of type IV preaxial polydactyly. Defects in GLI3 are the cause of acrocallosal syndrome (ACS). The antibody is specific to GLI3. At the molecular level, Gli3 is translated into a 190-kDa transcriptional activator (Gli3-190) that undergoes proteolytic processing into a truncated 83-kDa repressor (Gli3-83) lacking C-terminal activation domains. (PMID: 16705181)

实验方案

Product Specific Protocols
WB protocol for GLI3 antibody 28272-1-APDownload protocol
IHC protocol for GLI3 antibody 28272-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Bioengineered

All-trans-retinoic acid suppresses rat embryo hindlimb bud mesenchymal chondrogenesis by modulating HoxD9 expression

Authors - Quan Hong
View Article
humanWB

Medicine (Baltimore)

miR-200c targeting GLI3 inhibits cell proliferation and promotes apoptosis in non-small cell lung cancer cells

Authors - Xiangjun Yi
View Article