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GLI4 Polyclonal antibody

GLI4 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16551-1-AP

Print datasheet

Synonyms

Zinc finger protein GLI4, Protein HKR4, Krueppel-related zinc finger protein 4, HKR4, GLI family zinc finger 4

验证数据展示

  • WB analysis using 16551-1-AP

    WB analysis using 16551-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16551-1-AP (GLI4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 16551-1-AP

    IF Staining of A549 using 16551-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using GLI4 antibody (16551-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inJurkat cells, PC-3 cells, SH-SY5Y cells
Positive IF/ICC detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16551-1-AP targets GLI4 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen GLI4 fusion protein Ag9497 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GLI family zinc finger 4
Synonyms Zinc finger protein GLI4, Protein HKR4, Krueppel-related zinc finger protein 4, HKR4, GLI family zinc finger 4
Calculated Molecular Weight 376 aa, 41 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC014165
Gene Symbol GLI4
Gene ID (NCBI) 2738
RRIDAB_2109716
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10075
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GLI4 antibody 16551-1-APDownload protocol
IF protocol for GLI4 antibody 16551-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols